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Objectives <p>Bacteriophages are viruses that specifically target and kill bacteria. Bacteriophages are considered safe for humans, making them suitable for use in food applications Therefore, this study was conducted to isolate, characterize, and apply bacteriophages from the environment to control food spoilage bacteria.</p> Results <p>Bacteriophages were isolated by using <i>Bacillus cereus</i> (<i>B. cereus</i>) as reference host. We found bacteriophage BC-VP titers (1.16 ± 0.18 × 10<sup>9</sup> PFU/mL) and BC-AJ (1.72 ± 0.19 × 10<sup>8</sup> PFU/mL). Both bacteriophages were polyvalent, targeting not only <i>B. cereus</i>, but also lysing enteropathogenic <i>Escherichia coli</i> (EPEC) and enterotoxigenic <i>Escherichia coli</i> (ETEC) with different efficiencies. Bacteriophage BC-VP and BC-AJ miMOI were determined to be 0.01 and 1, respectively. Bacteriophage BC-VP morphology was analyzed using Transmission Electron Microscope (TEM) and categorized as <i>Myoviridae</i> family from the order of <i>Caudovirales</i>. Both bacteriophages showed significant bacterial reduction when applied to artificially contaminated cooked rice and pasteurized milk. The reduction number was higher in pasteurized milk at 28℃ for BC-VP (93%) and BC-AJ (90%). Molecular characterizations were performed and showed that BC-VP was 96.88% a virulent bacteriophage. Also, no sign of antibiotic resistance gene in BC-VP and genes related to lytic cycle such as putative tail lysin and tail fiber, were annotated.</p>

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Isolation and molecular characterization of bacteriophages isolated from lake water and their application in foods against Bacillus cereus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月2日

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  • Denny Rizkinata,
  • Vania Clarissa Kusnadi,
  • Diana Elizabeth Waturangi,
  • Adi Yulandi

摘要

Objectives

Bacteriophages are viruses that specifically target and kill bacteria. Bacteriophages are considered safe for humans, making them suitable for use in food applications Therefore, this study was conducted to isolate, characterize, and apply bacteriophages from the environment to control food spoilage bacteria.

Results

Bacteriophages were isolated by using Bacillus cereus (B. cereus) as reference host. We found bacteriophage BC-VP titers (1.16 ± 0.18 × 109 PFU/mL) and BC-AJ (1.72 ± 0.19 × 108 PFU/mL). Both bacteriophages were polyvalent, targeting not only B. cereus, but also lysing enteropathogenic Escherichia coli (EPEC) and enterotoxigenic Escherichia coli (ETEC) with different efficiencies. Bacteriophage BC-VP and BC-AJ miMOI were determined to be 0.01 and 1, respectively. Bacteriophage BC-VP morphology was analyzed using Transmission Electron Microscope (TEM) and categorized as Myoviridae family from the order of Caudovirales. Both bacteriophages showed significant bacterial reduction when applied to artificially contaminated cooked rice and pasteurized milk. The reduction number was higher in pasteurized milk at 28℃ for BC-VP (93%) and BC-AJ (90%). Molecular characterizations were performed and showed that BC-VP was 96.88% a virulent bacteriophage. Also, no sign of antibiotic resistance gene in BC-VP and genes related to lytic cycle such as putative tail lysin and tail fiber, were annotated.