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Objectives <p>The aim of this study was to determine the antibiofilm activity of bacteriophages, ETEC-phage-TG and BC-VP, isolated from freshwater lakes against biofilm of Enterohemorrhagic <i>Escherichia coli</i> and Enteropathogenic <i>Escherichia coli</i> on polystyrene and stainless-steel surfaces.</p> Results <p>Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 10<sup>5</sup> PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10<sup>− 2</sup>). Bacteriophage BC-VP had titer 8.0877 × 10<sup>4</sup> PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10<sup>− 3</sup>). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24&#xa0;h and 48&#xa0;h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24&#xa0;h and 48&#xa0;h) on stainless steel surface 33.21% and 43.94% respectively.</p>

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Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月14日

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  • King Archell,
  • Javier Stuart,
  • Diana Elizabeth Waturangi

摘要

Objectives

The aim of this study was to determine the antibiofilm activity of bacteriophages, ETEC-phage-TG and BC-VP, isolated from freshwater lakes against biofilm of Enterohemorrhagic Escherichia coli and Enteropathogenic Escherichia coli on polystyrene and stainless-steel surfaces.

Results

Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 105 PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10− 2). Bacteriophage BC-VP had titer 8.0877 × 104 PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10− 3). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24 h and 48 h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24 h and 48 h) on stainless steel surface 33.21% and 43.94% respectively.