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<p>Membrane filtration and Colilert assays are commonly used to quantify <i>Escherichia coli</i> levels in agricultural water. These methods have not been evaluated in test agricultural water (TAW), a formulation used for assessing chemical sanitizer effectiveness. Quantitative recovery of <i>E. coli</i> in TAW may be affected by turbidity levels. TAW was formulated with a pH value of 6.5 at two turbidity levels (0 and 50 NTU). Pan-susceptible and cefotaxime-resistant <i>E. coli</i> strains were added separately to TAW (200 CFU/100 mL). Inoculated TAW was either (1) filtered using a 0.45&#xa0;μm membrane filter (MF) and placed onto Tryptone Bile X-Glucuronide (TBX) agar or CHROMagar ECC (ECC), or (2) tested by IDEXX Quanti-Tray/2000 Colilert. Recovery percentages of <i>E. coli</i> at both turbidity levels were similar on TBX and ECC, but pan-susceptible <i>E. coli</i> ARS C101 in TAW at 50 NTU on TBX was not quantifiable. For Colilert assays, the recovery percentages of <i>E. coli</i> from 0 NTU and 50 NTU TAW were between 93.4 ± 4.4% and 127.0 ± 10.5%. Overall, ECC and Colilert provided quantifiable results for recovery of pan-susceptible and cefotaxime-resistant <i>E. coli</i> from artificial agricultural water at different turbidity levels. Higher levels of turbidity affected the ability to quantify <i>E. coli</i> by MF with TBX and recovery by Colilert.</p>

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Evaluating the recovery of pan-susceptible and antibiotic-resistant Escherichia coli in synthetic test agricultural water using membrane filtration and colilert methods

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月20日

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  • Zirui Ray Xiong,
  • Ajani A. Brooks,
  • Ellen Gabriel,
  • Alan Gutierrez,
  • Shayla B. Johnson,
  • Cheryl East,
  • Lisa M. Durso,
  • Elica Moss,
  • Manan Sharma

摘要

Membrane filtration and Colilert assays are commonly used to quantify Escherichia coli levels in agricultural water. These methods have not been evaluated in test agricultural water (TAW), a formulation used for assessing chemical sanitizer effectiveness. Quantitative recovery of E. coli in TAW may be affected by turbidity levels. TAW was formulated with a pH value of 6.5 at two turbidity levels (0 and 50 NTU). Pan-susceptible and cefotaxime-resistant E. coli strains were added separately to TAW (200 CFU/100 mL). Inoculated TAW was either (1) filtered using a 0.45 μm membrane filter (MF) and placed onto Tryptone Bile X-Glucuronide (TBX) agar or CHROMagar ECC (ECC), or (2) tested by IDEXX Quanti-Tray/2000 Colilert. Recovery percentages of E. coli at both turbidity levels were similar on TBX and ECC, but pan-susceptible E. coli ARS C101 in TAW at 50 NTU on TBX was not quantifiable. For Colilert assays, the recovery percentages of E. coli from 0 NTU and 50 NTU TAW were between 93.4 ± 4.4% and 127.0 ± 10.5%. Overall, ECC and Colilert provided quantifiable results for recovery of pan-susceptible and cefotaxime-resistant E. coli from artificial agricultural water at different turbidity levels. Higher levels of turbidity affected the ability to quantify E. coli by MF with TBX and recovery by Colilert.