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Background <p>Human adenoviruses (HAdVs) cause various diseases, and they frequently undergo recombination. As adenoviruses are susceptible to recombination, whole-genome sequencing and analysis are essential approaches to understanding viral properties. In the present study, we performed whole-genome sequencing to characterize adenoviruses and assess the local epidemic genotypes of adenovirus-related diseases in Kobe, Japan.</p> Methods <p>Eighty HAdV cell culture-positive strains isolated from clinical specimens in Kobe City, Japan, between 2018 and 2022 were used. Whole-genome sequencing, phylogenetic analysis, and recombination analysis were performed.</p> Results <p>Disease-specific HAdV species were detected, with species B and C being the primary species detected for pharyngoconjunctival fever and species F for infectious gastroenteritis. All species B strains belonged to the HAdV-3 genotype. Multiple genotypes were detected in species C, including five strains in which the new genotype, P85H5F5, was identified in pharyngoconjunctival fever specimens.</p> Conclusions <p>Whole-genome analysis of HAdV is an important approach not only for understanding local epidemics, but also for monitoring the emergence of recombinant genotypes.</p>

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Whole genome-based surveillance for human adenovirus-related diseases in Kobe City, Japan, 2018–2022

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月15日

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  • Yoshihiko Tanimoto,
  • Minori Ohyama,
  • Erika Ito,
  • Kyoko Akiyoshi,
  • Yuka Onishi,
  • Ai Mori,
  • Ryohei Nomoto

摘要

Background

Human adenoviruses (HAdVs) cause various diseases, and they frequently undergo recombination. As adenoviruses are susceptible to recombination, whole-genome sequencing and analysis are essential approaches to understanding viral properties. In the present study, we performed whole-genome sequencing to characterize adenoviruses and assess the local epidemic genotypes of adenovirus-related diseases in Kobe, Japan.

Methods

Eighty HAdV cell culture-positive strains isolated from clinical specimens in Kobe City, Japan, between 2018 and 2022 were used. Whole-genome sequencing, phylogenetic analysis, and recombination analysis were performed.

Results

Disease-specific HAdV species were detected, with species B and C being the primary species detected for pharyngoconjunctival fever and species F for infectious gastroenteritis. All species B strains belonged to the HAdV-3 genotype. Multiple genotypes were detected in species C, including five strains in which the new genotype, P85H5F5, was identified in pharyngoconjunctival fever specimens.

Conclusions

Whole-genome analysis of HAdV is an important approach not only for understanding local epidemics, but also for monitoring the emergence of recombinant genotypes.