Scholarlink

Objective <p>Cadmium (Cd) is a highly toxic metal element and a carcinogen to humans. Rice is prone to taking up Cd from paddy fields and accumulating it in grain, which raises health concerns for rice consumers. OsNramp5 is a major transporter for Cd and manganese (Mn) uptake in rice, whereas OsHMA3 is a tonoplast-localized transporter involved in Cd detoxification in vacuoles. In this study, we compared the efficiency of <i>OsNramp5</i> knockout mutation and <i>OsHMA3</i> overexpression in reducing Cd content in rice grain.</p> Results <p>The grain Cd content of the <i>OsNramp5</i> knockout mutants was significantly lower than that of the wild-type rice T5105. However, these mutants still had much higher grain Cd content than the previously reported <i>OsNramp5</i> mutants or the <i>OsHMA3</i> overexpression lines developed in our previous study. Pyramiding the <i>OsNramp5</i> mutant allele and the <i>OsHMA3</i> transgene in a single line did not result in an additional reduction in grain Cd content. The <i>OsNramp5</i> gene in T5105 has a haplotype II promoter, and its knockout mutation also partially reduced Mn content in rice grain. Our results demonstrate that <i>OsHMA3</i> overexpression works more efficiently in generating low-Cd rice grain than <i>OsNramp5</i> knockout mutation without affecting Mn uptake in rice.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月5日

查看全文
  • Yuejing Gui,
  • Joanne Teo,
  • Dongsheng Tian,
  • Raji Mohan,
  • Zhongchao Yin

摘要

Objective

Cadmium (Cd) is a highly toxic metal element and a carcinogen to humans. Rice is prone to taking up Cd from paddy fields and accumulating it in grain, which raises health concerns for rice consumers. OsNramp5 is a major transporter for Cd and manganese (Mn) uptake in rice, whereas OsHMA3 is a tonoplast-localized transporter involved in Cd detoxification in vacuoles. In this study, we compared the efficiency of OsNramp5 knockout mutation and OsHMA3 overexpression in reducing Cd content in rice grain.

Results

The grain Cd content of the OsNramp5 knockout mutants was significantly lower than that of the wild-type rice T5105. However, these mutants still had much higher grain Cd content than the previously reported OsNramp5 mutants or the OsHMA3 overexpression lines developed in our previous study. Pyramiding the OsNramp5 mutant allele and the OsHMA3 transgene in a single line did not result in an additional reduction in grain Cd content. The OsNramp5 gene in T5105 has a haplotype II promoter, and its knockout mutation also partially reduced Mn content in rice grain. Our results demonstrate that OsHMA3 overexpression works more efficiently in generating low-Cd rice grain than OsNramp5 knockout mutation without affecting Mn uptake in rice.