Background <p>Amphibian populations are declining worldwide, in part due to diseases caused by viruses, fungi, andparasites. Giant Anuran Trypanosomes (GATs) are parasites that affect frogs worldwide and require a vector to betransmitted.<i>Culex territans</i>is an amphibian-feeding mosquito suspected to be a vector of trypanosomes, but this hasnot previously been confirmed.</p> Methods <p>In this study, we tested blood-fedCx. territansand blood from their primary anuran hosts,<i>Rana clamitans</i>and<i>R</i>. <i>catesbeiana</i>, in southwest Virginia. Additionally, we tested potential routes of transmission from the mosquito tothe frog.</p> Results <p>We found trypanosomes present in both mosquitoes and anurans and found trypanosomes present in thefeces 2 days after being blood fed on infected frogs, as well as in the body and saliva 14 days post-feeding.</p> Conclusions <p>Overall, this study contributes to our knowledge of the GAT epidemiology and the roleCx. territansmightplay in their transmission.</p> Graphical Abstract <p></p>