Scholarlink

Background <p>The immunofluorescence antibody test (IFAT) is a serological diagnostic technique used to quantify serum antibodies generated in response to exposure to various pathogens, such as <i>Leishmania infantum</i>. Retrospective analysis of previously collected frozen samples is highly valuable for clinical and research purposes. The primary aim of this study was to evaluate the impact of long-term frozen storage of canine plasma samples on the IFAT-based serological diagnosis of <i>L. infantum</i> exposure.</p> Methods <p>A total of 189 frozen plasma samples from dogs stored at −20&#xa0;°C for 5, 10 or 20&#xa0;years, which had previously been tested for <i>L. infantum</i> exposure via IFAT (IgG), were reanalysed to assess the concordance between past and current qualitative and quantitative results.</p> Results <p>The qualitative agreement between the former and current IFATs was 92.1%. The samples from 20&#xa0;years prior presented the greatest increase in negative samples in the second analysis (from 28.6 to 39.7%). A strong positive correlation was observed between the quantitative measurements of the past and present across all three groups. The exact quantitative agreement was 48.7%.</p> Conclusions <p>The results of this study indicate that freezing at −20&#xa0;°C is a good technique for prolonged storage of samples for the detection of <i>L. infantum</i> exposure in dogs, as the qualitative IFAT result is not significantly altered. This finding is of particular interest both for clinical endeavours and for future research in this field.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluating the stability of antibody titres against Leishmania infantum determined by IFAT in long-term stored canine frozen samples

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月3日

查看全文
  • Patricia Olmeda,
  • David Díaz-Regañón,
  • Alejandra Villaescusa,
  • Inmaculada Amusategui,
  • Miguel A. Tesouro,
  • Fernando Rodríguez-Franco,
  • Mercedes García-Sancho,
  • Daniel Martín-Fraile,
  • Ángel Sainz

摘要

Background

The immunofluorescence antibody test (IFAT) is a serological diagnostic technique used to quantify serum antibodies generated in response to exposure to various pathogens, such as Leishmania infantum. Retrospective analysis of previously collected frozen samples is highly valuable for clinical and research purposes. The primary aim of this study was to evaluate the impact of long-term frozen storage of canine plasma samples on the IFAT-based serological diagnosis of L. infantum exposure.

Methods

A total of 189 frozen plasma samples from dogs stored at −20 °C for 5, 10 or 20 years, which had previously been tested for L. infantum exposure via IFAT (IgG), were reanalysed to assess the concordance between past and current qualitative and quantitative results.

Results

The qualitative agreement between the former and current IFATs was 92.1%. The samples from 20 years prior presented the greatest increase in negative samples in the second analysis (from 28.6 to 39.7%). A strong positive correlation was observed between the quantitative measurements of the past and present across all three groups. The exact quantitative agreement was 48.7%.

Conclusions

The results of this study indicate that freezing at −20 °C is a good technique for prolonged storage of samples for the detection of L. infantum exposure in dogs, as the qualitative IFAT result is not significantly altered. This finding is of particular interest both for clinical endeavours and for future research in this field.

Graphical Abstract