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Background <p>Some species of <i>Culicoides</i> (Diptera, Ceratopogonidae) are major vectors for arboviruses, and Yunnan Province is a key area for arbovirus prevalence in China. Therefore, this study attempts to search for potential <i>Culicoides</i> vectors for the common orbiviruses bluetongue virus (BTV), epizootic hemorrhagic disease virus (EHDV), Palyam virus (PALV) and Tibet orbivirus (TIBOV) in Yunnan Province, China.</p> Methods <p><i>Culicoides</i> specimens were collected from 16 counties in Yunnan Province, China, using UV traps and tested for BTV, EHDV, PALV and TIBOV through one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A total of 543 conspecific pools of <i>Culicoides</i> containing 9895 specimens were tested.</p> Results <p>A total of 46 species belonging to 8 subgenera and 2 groups were recognized. A total of 19 species and a <i>Culicoides</i> subgenus <i>Trithecoides</i> complex were tested using RT-qPCR. One pool of <i>Culicoides shortti</i> Smith &amp; Swaminath and one pool of <i>Culicoides orientalis</i> Macfie tested positive for BTV, one pool of <i>Culicoides oxystoma</i> Kieffer tested positive for PALV, and four pools of <i>Culicoides jacobsoni</i> Macfie tested positive for TIBOV. All the tested samples were negative for EHDV, and all the tested <i>C.</i> subgenus <i>Trithecoides</i> midges were negative for any virus.</p> Conclusions <p><i>Culicoides shortti</i> was identified as a potential BTV vector for the first time. <i>Culicoides jacobsoni</i> was confirmed as a potential TIBOV vector and <i>C. orientalis</i> as a potential BTV vector. <i>Culicoides oxystoma</i> was also shown to be a natural carrier of PALV using the RT-qPCR method.</p> Graphical Abstract <p></p>

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Searching for potential Culicoides vectors of four orbiviruses in Yunnan Province, China

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月24日

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  • Zhan Hong Li,
  • Yi Nan Wang,
  • Jia Ming Deng,
  • Le Li,
  • Lian Jiang Yang,
  • Xinq Qiang Chen,
  • Wen Hua Wang,
  • Fu You Lu,
  • Zhong Jie Tang,
  • Dong Mei Wang,
  • Ying Liang Duan

摘要

Background

Some species of Culicoides (Diptera, Ceratopogonidae) are major vectors for arboviruses, and Yunnan Province is a key area for arbovirus prevalence in China. Therefore, this study attempts to search for potential Culicoides vectors for the common orbiviruses bluetongue virus (BTV), epizootic hemorrhagic disease virus (EHDV), Palyam virus (PALV) and Tibet orbivirus (TIBOV) in Yunnan Province, China.

Methods

Culicoides specimens were collected from 16 counties in Yunnan Province, China, using UV traps and tested for BTV, EHDV, PALV and TIBOV through one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A total of 543 conspecific pools of Culicoides containing 9895 specimens were tested.

Results

A total of 46 species belonging to 8 subgenera and 2 groups were recognized. A total of 19 species and a Culicoides subgenus Trithecoides complex were tested using RT-qPCR. One pool of Culicoides shortti Smith & Swaminath and one pool of Culicoides orientalis Macfie tested positive for BTV, one pool of Culicoides oxystoma Kieffer tested positive for PALV, and four pools of Culicoides jacobsoni Macfie tested positive for TIBOV. All the tested samples were negative for EHDV, and all the tested C. subgenus Trithecoides midges were negative for any virus.

Conclusions

Culicoides shortti was identified as a potential BTV vector for the first time. Culicoides jacobsoni was confirmed as a potential TIBOV vector and C. orientalis as a potential BTV vector. Culicoides oxystoma was also shown to be a natural carrier of PALV using the RT-qPCR method.

Graphical Abstract