<p>Extracellular Vesicles (EVs), two types of different size: microvesicles and exosomes, generated by all types of cells. EV interactions with lysosomes and/or autophagosomes, induce establishment of combined forms. EVs are composed by a surface membrane of lipid bilayer rich of specific proteins around a cargo with distinct lipids and proteins together with various forms of RNA. This review illustrates the whole life of EVs, from their intracellular generation followed by their release to their extracellular space. The ensuing EV navigations lead to binding/penetration into cells and solid/fluid organs, selected based on specific markers, receptors and metabolism. Modified EVs undergo release with binding to other vesicles accompanied by regeneration of recycling to appropriate cells, at some distance or even far away from their origin/establishments. By action with interactive cells, EVs may induce either health processes or various events of diseases, often employed for diagnosis and interest of patients. By acting especially on stem cells of mesenchymal and cancer nature, EVs induce changes of brain, heart, immunology and other diseases, especially cancer. Upon appropriate engineering various EVs, possibly converted into artificial nanovesicles, undergo loading and traffic of drugs and nanomaterials. These and other factors strengthen the EV therapy by ensuing dynamics in critical cancer conditions. A fraction of EV investigation can be converted into established medical employment by confirmed clinical trials. At present, established conversions are progressed towards direct treatments. Ongoing innovative/promising developments are planned, established for future research and therapies.</p><p><?noindent??><b>Clinical trial number</b> Not applicable.</p>