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<p>High-throughput sequencing with chemical labeling enables robust, transcriptome-wide detection of RNA modifications at single-nucleotide resolution. However, these methods typically require substantial RNA amounts due to harsh treatments. We introduce Uli-epic, an innovative library construction strategy that enables profiling epitranscriptomic modifications using 100&#xa0;pg to 1&#xa0;ng of RNA. Utilizing Uli-epic BID-seq, we investigate pseudouridine (Ψ) sites in neural stem cells and sperm RNA from wild-type and fetal growth restriction mice, using only 500&#xa0;pg of rRNA-depleted RNA. Uli-epic GLORI quantifies m<sup>6</sup>A in sperm and neural stem cells from wild-type and fetal growth restriction mice, using 10&#xa0;ng of rRNA-depleted RNA.</p> Graphical Abstract <p></p>

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Uli-epic: profiling RNA modifications from ultra-low input samples

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年11月11日

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  • Weizhi He,
  • Chu Xu,
  • Wen Chen,
  • Xiao Jiang,
  • Yuhang Wang,
  • Yong Peng,
  • Shujuan Chang,
  • Wei Zhu,
  • Jiuhong Kang,
  • Lulu Hu

摘要

High-throughput sequencing with chemical labeling enables robust, transcriptome-wide detection of RNA modifications at single-nucleotide resolution. However, these methods typically require substantial RNA amounts due to harsh treatments. We introduce Uli-epic, an innovative library construction strategy that enables profiling epitranscriptomic modifications using 100 pg to 1 ng of RNA. Utilizing Uli-epic BID-seq, we investigate pseudouridine (Ψ) sites in neural stem cells and sperm RNA from wild-type and fetal growth restriction mice, using only 500 pg of rRNA-depleted RNA. Uli-epic GLORI quantifies m6A in sperm and neural stem cells from wild-type and fetal growth restriction mice, using 10 ng of rRNA-depleted RNA.

Graphical Abstract