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<p>The aim of the present study was to determine whether <i>L. monocytogenes</i> bacteria from an invasive case of listeriosis cultured in a BacT/ALERT FA bottle in 1999 have survived, and if so, whether their pulsed-field gel electrophoresis (PFGE) profiles have changed. In 2024, after more than 25&#xa0;years in a refrigerator, <i>L. monocytogenes</i> was isolated in pure culture from the BacT/ALERT FA bottle. All 100 selected isolates showed the same by PFGE profile with the restriction enzyme <i>Asc</i> I as the <i>L. monocytogenes</i> isolates did in 1999. <i>L. monocytogenes</i> can apparently survive for a long time in a nutrient-poor environment, and this must be taken into account by the food industry. Persistence in clinical samples has rarely been reported; however, several authors report persistence of <i>L. monocytogenes</i> in the food industry, with bacteria ending up in final products.</p>

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Listeria monocytogenes isolated from a human case of invasive listeriosis survived more than 25 years in a blood culture bottle

  • 期刊论文

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  • 发表日期：

    2026年6月29日

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  • Wilhelm Tham,
  • Hannu Korkeala,
  • Gloria Lopez Valladares,
  • Marie-Louise Danielsson-Tham

摘要

The aim of the present study was to determine whether L. monocytogenes bacteria from an invasive case of listeriosis cultured in a BacT/ALERT FA bottle in 1999 have survived, and if so, whether their pulsed-field gel electrophoresis (PFGE) profiles have changed. In 2024, after more than 25 years in a refrigerator, L. monocytogenes was isolated in pure culture from the BacT/ALERT FA bottle. All 100 selected isolates showed the same by PFGE profile with the restriction enzyme Asc I as the L. monocytogenes isolates did in 1999. L. monocytogenes can apparently survive for a long time in a nutrient-poor environment, and this must be taken into account by the food industry. Persistence in clinical samples has rarely been reported; however, several authors report persistence of L. monocytogenes in the food industry, with bacteria ending up in final products.