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Background <p>Osteoarthritis (OA) significantly impairs the quality of life of middle-aged and elderly individuals. MicroRNAs (miRNAs) are known to play key regulatory roles in OA progression.</p> Aims <p>To investigate the potential role mechanisms of miR-409-5p in OA.</p> Methods <p>This study enrolled 93 OA patients and 75 non-OA controls. The expression levels of miR-409-5p, DLST, and OA-related markers were measured using RT-qPCR or Western Blot. The targeting relationship between miR-409-5p and DLST was verified by dual luciferase reporter assays. Cell viability was assessed with the CCK-8 assay, and inflammatory cytokine concentrations were measured via ELISA.</p> Results <p>Serum miR-409-5p expression was higher in OA patients than in controls and showed good diagnostic value for OA. Inhibiting miR-409-5p suppressed IL-1β-induced viability in CHON-001 cells, promoted aggrecan expression, suppressed MMP13 expression, and reduced secretion of IL-6 and IL-8. MiR-409-5p directly targeted DLST, and their expression levels were negatively correlated. Knockdown of DLST abolished the beneficial effects of miR-409-5p inhibition on cell viability, extracellular matrix degradation, and inflammatory responses.</p> Conclusions <p>MiR-409-5p was highly expressed in OA and may promote disease progression by targeting DLST.</p>

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Elevated miR-409-5p may promote the progression of osteoarthritis by targeting DLST as a potential biomarker function of miR-409-5p in osteoarthritis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月27日

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  • Jinpeng Zheng,
  • Qingyi Wang,
  • Qin Yu,
  • Baohui Zhao,
  • Yu Zhang,
  • Heng Zhang

摘要

Background

Osteoarthritis (OA) significantly impairs the quality of life of middle-aged and elderly individuals. MicroRNAs (miRNAs) are known to play key regulatory roles in OA progression.

Aims

To investigate the potential role mechanisms of miR-409-5p in OA.

Methods

This study enrolled 93 OA patients and 75 non-OA controls. The expression levels of miR-409-5p, DLST, and OA-related markers were measured using RT-qPCR or Western Blot. The targeting relationship between miR-409-5p and DLST was verified by dual luciferase reporter assays. Cell viability was assessed with the CCK-8 assay, and inflammatory cytokine concentrations were measured via ELISA.

Results

Serum miR-409-5p expression was higher in OA patients than in controls and showed good diagnostic value for OA. Inhibiting miR-409-5p suppressed IL-1β-induced viability in CHON-001 cells, promoted aggrecan expression, suppressed MMP13 expression, and reduced secretion of IL-6 and IL-8. MiR-409-5p directly targeted DLST, and their expression levels were negatively correlated. Knockdown of DLST abolished the beneficial effects of miR-409-5p inhibition on cell viability, extracellular matrix degradation, and inflammatory responses.

Conclusions

MiR-409-5p was highly expressed in OA and may promote disease progression by targeting DLST.