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Background <p>Therapeutic strategies that focus on chondrocyte protection and matrix repair may represent a key direction for delaying the progression of osteoarthritis (OA).</p> Aim <p>To evaluate miR-151a-3p as a biomarker for the diagnosis of OA and its potential mechanism of action.</p> Materials and methods <p>RT-qPCR was used to determine miR-151a-3p and SOX9 expression levels in OA patients and lipopolysaccharide-induced OA (LPS-OA) cell model. The receiver operating characteristic (ROC) curve was used to analyze the clinical value of miR-151a-3p in the diagnosis of OA. A cell function experiment was conducted to investigate the impact of miR-151a-3p on cell viability and apoptosis levels in the LPS-induced OA cell model. Genecards and starBase were used to screen the target genes of miR-151a-3p. The dual luciferase reporter assay was used to verify the interaction between miR-151a-3p and SOX9.</p> Results <p>The expression of miR-151a-3p was up-regulated in synovial fluid of OA patients and LPS-OA cell model. The ROC curve analysis and logistic regression analysis demonstrated that miR-151a-3p could be a potential biomarker for the diagnosis of OA and exhibited significant diagnostic value. Genecards and starBase databases screened 6 potential targets of miR-151a-3p. Dual luciferase reporter assay demonstrated that miR-151a-3p targeted SOX9. Cell function experiments demonstrated that suppressed miR-151a-3p can upregulate SOX9 expression, thereby enhancing LPS-OA cell activity, reducing apoptosis, and increasing extracellular matrix (ECM) production.</p> Conclusion <p>MiR-151a-3p could be a biomarker for the diagnosis of OA and has high diagnostic value.</p>

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Analysis of miR-151a-3p as a biomarker of osteoarthritis and its clinical value

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月24日

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  • Xuchen Liu,
  • Min Zhou,
  • Dejian Yang,
  • Lihua Gong,
  • Xiong Chen,
  • Zhiwei Zeng,
  • Yingxuan Huang

摘要

Background

Therapeutic strategies that focus on chondrocyte protection and matrix repair may represent a key direction for delaying the progression of osteoarthritis (OA).

Aim

To evaluate miR-151a-3p as a biomarker for the diagnosis of OA and its potential mechanism of action.

Materials and methods

RT-qPCR was used to determine miR-151a-3p and SOX9 expression levels in OA patients and lipopolysaccharide-induced OA (LPS-OA) cell model. The receiver operating characteristic (ROC) curve was used to analyze the clinical value of miR-151a-3p in the diagnosis of OA. A cell function experiment was conducted to investigate the impact of miR-151a-3p on cell viability and apoptosis levels in the LPS-induced OA cell model. Genecards and starBase were used to screen the target genes of miR-151a-3p. The dual luciferase reporter assay was used to verify the interaction between miR-151a-3p and SOX9.

Results

The expression of miR-151a-3p was up-regulated in synovial fluid of OA patients and LPS-OA cell model. The ROC curve analysis and logistic regression analysis demonstrated that miR-151a-3p could be a potential biomarker for the diagnosis of OA and exhibited significant diagnostic value. Genecards and starBase databases screened 6 potential targets of miR-151a-3p. Dual luciferase reporter assay demonstrated that miR-151a-3p targeted SOX9. Cell function experiments demonstrated that suppressed miR-151a-3p can upregulate SOX9 expression, thereby enhancing LPS-OA cell activity, reducing apoptosis, and increasing extracellular matrix (ECM) production.

Conclusion

MiR-151a-3p could be a biomarker for the diagnosis of OA and has high diagnostic value.