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Objective <p>To explore the role and mechanism of miR-122-5p in the process of fracture healing following a Pilon fracture, as well as to further assess the clinical significance of this molecule as a potential diagnostic marker for fracture nonunion.</p> Methods <p>The expression levels of miR-122-5p were examined in patients with Pilon fracture healed and nonunion, respectively. ROC analysis was employed to evaluate the diagnostic value of miR-122-5p in identifying fracture nonunion, while logistic regression identified its risk factors. The effects of miR-122-5p on the proliferation, apoptosis, and osteogenic differentiation markers of osteoblasts were analyzed by CCK-8 assays, flow cytometry, RT-qPCR, and ALP assay kit. Additionally, DLR and RIP assays validated miR-122-5p targeting PDCD4.</p> Results <p>Serum miR-122-5p was significantly under-expressed in patients with fracture nonunion, whereas PDCD4 exhibits a notable over-expressed. Serum miR-122-5p can predict non-union of fractures, with a sensitivity of 90.82% and a specificity of 78.33%. Low miR-122-5p expression is a potential risk factor for fracture nonunion. The overexpression of miR-122-5p enhanced the proliferation ability of osteoblasts, inhibited cell apoptosis, and upregulated the expressions of ALP, OCN, and RUNX2; knockdown of miR-122-5p elicits opposite effect. PDCD4 is a direct target of miR-122-5p. The osteoblast dysfunction induced by miR-122-5p inhibitors can be reversed by down-regulating PDCD4.</p> Conclusion <p>Diminished miR-122-5p emerge as a potential prognostic indicator for nonunion in Pilon fractures. miR-122-5p accelerates the healing of Pilon fractures by targeting and inhibiting PDCD4.</p>

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The role and regulatory mechanism of miR-122-5p in the process of pilon fracture healing

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月26日

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  • Yang Zhang,
  • Yingnan Li,
  • Min Zhou,
  • Lichang Yang

摘要

Objective

To explore the role and mechanism of miR-122-5p in the process of fracture healing following a Pilon fracture, as well as to further assess the clinical significance of this molecule as a potential diagnostic marker for fracture nonunion.

Methods

The expression levels of miR-122-5p were examined in patients with Pilon fracture healed and nonunion, respectively. ROC analysis was employed to evaluate the diagnostic value of miR-122-5p in identifying fracture nonunion, while logistic regression identified its risk factors. The effects of miR-122-5p on the proliferation, apoptosis, and osteogenic differentiation markers of osteoblasts were analyzed by CCK-8 assays, flow cytometry, RT-qPCR, and ALP assay kit. Additionally, DLR and RIP assays validated miR-122-5p targeting PDCD4.

Results

Serum miR-122-5p was significantly under-expressed in patients with fracture nonunion, whereas PDCD4 exhibits a notable over-expressed. Serum miR-122-5p can predict non-union of fractures, with a sensitivity of 90.82% and a specificity of 78.33%. Low miR-122-5p expression is a potential risk factor for fracture nonunion. The overexpression of miR-122-5p enhanced the proliferation ability of osteoblasts, inhibited cell apoptosis, and upregulated the expressions of ALP, OCN, and RUNX2; knockdown of miR-122-5p elicits opposite effect. PDCD4 is a direct target of miR-122-5p. The osteoblast dysfunction induced by miR-122-5p inhibitors can be reversed by down-regulating PDCD4.

Conclusion

Diminished miR-122-5p emerge as a potential prognostic indicator for nonunion in Pilon fractures. miR-122-5p accelerates the healing of Pilon fractures by targeting and inhibiting PDCD4.