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Background <p>Spinal cord injury (SCI) can cause severe motor and sensory deficits below the injury site. Studies have highlighted the multifaceted regulatory roles of miR-29c-3p in neuronal development and function; however, its role in SCI remains unclear.</p> Methods <p>In this research 105 healthy controls and 159 patients with SCI were recruited. The miR-29c-3p and BRD4 levels in serum or cells were estimated by RT-qPCR. The diagnostic performance of miR-29c-3p was assessed by ROC curve. Moreover, cell viability and apoptosis were measured via CCK8 and flow cytometry. The pro-inflammatory cytokines were examined by ELISA assay. The pathological condition of SCI was modeled with LPS-induced PC12 cells. The target relationship between miR-29c-3p and BRD4 was verified by the dual-luciferase reporter assay.</p> Results <p>Serum miR-29c-3p was remarkedly decreased in the SCI population and showed high clinical diagnostic performance. Under pathological conditions, the upregulation of miR-29c-3p effectively reversed the significant reduction in cell viability and the increase in apoptosis rate. Moreover, enhanced pro-inflammatory cytokines including TNF-α, IL-6 and IL-1β were also attenuated by the upregulation of miR-29c-3p. BRD4 was identified as a target of miR-29c-3p and negatively regulated by miR-29c-3p.</p> Conclusions <p>A decrease in miR-29c-3p is a promising diagnostic indicator for SCI, and downregulation of miR-29c-3p accelerates the progression of SCI by targeting BRD4.</p>

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miR-29c-3p downregulation accelerates spinal cord injury progression by targeting BRD4

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月25日

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  • Dawei Wang,
  • Xiaona Wang,
  • Yingshuang Wu,
  • Sina Qi,
  • Haifeng Hu,
  • Jifei Guo,
  • Yi Luo

摘要

Background

Spinal cord injury (SCI) can cause severe motor and sensory deficits below the injury site. Studies have highlighted the multifaceted regulatory roles of miR-29c-3p in neuronal development and function; however, its role in SCI remains unclear.

Methods

In this research 105 healthy controls and 159 patients with SCI were recruited. The miR-29c-3p and BRD4 levels in serum or cells were estimated by RT-qPCR. The diagnostic performance of miR-29c-3p was assessed by ROC curve. Moreover, cell viability and apoptosis were measured via CCK8 and flow cytometry. The pro-inflammatory cytokines were examined by ELISA assay. The pathological condition of SCI was modeled with LPS-induced PC12 cells. The target relationship between miR-29c-3p and BRD4 was verified by the dual-luciferase reporter assay.

Results

Serum miR-29c-3p was remarkedly decreased in the SCI population and showed high clinical diagnostic performance. Under pathological conditions, the upregulation of miR-29c-3p effectively reversed the significant reduction in cell viability and the increase in apoptosis rate. Moreover, enhanced pro-inflammatory cytokines including TNF-α, IL-6 and IL-1β were also attenuated by the upregulation of miR-29c-3p. BRD4 was identified as a target of miR-29c-3p and negatively regulated by miR-29c-3p.

Conclusions

A decrease in miR-29c-3p is a promising diagnostic indicator for SCI, and downregulation of miR-29c-3p accelerates the progression of SCI by targeting BRD4.