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Background <p>Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a porcine enteric coronavirus that induces watery diarrhea in pigs, causing substantial economic losses in the swine industry. While the molecular and serological epidemiology of SADS-CoV in China has been extensively studied, comprehensive epidemiological studies assessing its prevalence outside China are lacking.</p> Results <p>In this study, an indirect enzyme-linked immunosorbent assay based on the SADS-CoV N protein (N-iELISA) was developed to evaluate the seroprevalence of SADS-CoV in Korean pig herds. The optimal conditions for N-iELISA were determined through checkerboard titration of serum samples verified via western blotting. The assay showed sufficient specificity and reproducibility, with a cutoff value of 0.484. A total of 540 field samples collected from pig herds across nine provinces in Korea were subsequently tested using the N-iELISA. The findings revealed an overall seroprevalence of SADS-CoV in Korea of 8.70%.</p> Conclusions <p>These results indicate that the N-iELISA is a reliable tool for seroepidemiological studies of SADS-CoV and suggest that the seroprevalence of SADS-CoV in the Korean pig population is relatively low.</p>

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Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibody

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月20日

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  • Yu-Jeong Jang,
  • Nam Phuong Le,
  • Eun-Song Lee,
  • Min-Chae Kim,
  • Tae-Kyung Chang,
  • Jung-Eun Park

摘要

Background

Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a porcine enteric coronavirus that induces watery diarrhea in pigs, causing substantial economic losses in the swine industry. While the molecular and serological epidemiology of SADS-CoV in China has been extensively studied, comprehensive epidemiological studies assessing its prevalence outside China are lacking.

Results

In this study, an indirect enzyme-linked immunosorbent assay based on the SADS-CoV N protein (N-iELISA) was developed to evaluate the seroprevalence of SADS-CoV in Korean pig herds. The optimal conditions for N-iELISA were determined through checkerboard titration of serum samples verified via western blotting. The assay showed sufficient specificity and reproducibility, with a cutoff value of 0.484. A total of 540 field samples collected from pig herds across nine provinces in Korea were subsequently tested using the N-iELISA. The findings revealed an overall seroprevalence of SADS-CoV in Korea of 8.70%.

Conclusions

These results indicate that the N-iELISA is a reliable tool for seroepidemiological studies of SADS-CoV and suggest that the seroprevalence of SADS-CoV in the Korean pig population is relatively low.