Background <p>The roles of pharmacists have changed in recent times, with pharmacists advancing public health through immunization.</p> Objective <p>This study aimed to evaluate community pharmacists’ knowledge, attitude and role in Hepatitis B immunization in Nigeria.</p> Methods <p>A cross-sectional descriptive study was conducted among 49 registered community pharmacists in Anambra state. Convenience sampling technique was employed. A structured questionnaire was adapted as instrument for data collection. Data were analyzed using SPSS version 23 and summarized using descriptive statistics: frequency, percentage, and mean. Scores obtained were compared using ANOVA and Independent Student’s t-test. The relationship between the sociodemographic characteristics of the respondents and their role in Hepatitis B vaccination was assessed using the Chi-square test. Statistical significance was established at p &lt; 0.05.</p> Results <p>More females, 31 (63.3%), than males participated in the study. Majority of the pharmacists 46 (93.3%), had B.Pharm. as highest qualification, while 40 (81.6%) were within the first five years of practice. Forty-five (91.8%) respondents stated that asymptomatic people can infect others while 26(53.1%) respondents have not been tested for Hepatitis B infection. Although 45 (91.8%) pharmacists provide immunization services in their pharmacies, only one (2%) pharmacist administer Hepatitis B vaccine to clients. Community pharmacists’ provision of immunization services and their role in Hepatitis B immunization had no statistically significant relationship with their sociodemographic characteristics.</p> Conclusion <p>The community pharmacists had good knowledge but suboptimal attitude to Hepatitis B immunization. Their role involved educating clients on Hepatitis B immunization. Adequate efforts should be made to empower community pharmacists in other areas.</p>