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Background <p>Analyzing the content of immune cell-derived extracellular vesicles (EVs) may reveal biomarkers that elucidate the mechanisms through which infection negatively affects outcomes in patients with intracerebral hemorrhage (ICH).</p> Methods <p>A prospective observational study in patients with acute ICH classified by the occurrence of in-hospital infection within 7 days and outcomes at 6 months, good outcome defined as an improvement of &gt; 10 points or &gt; 50% in NIHSS score and a mRS score 0–2. Immune cell-derived EVs were obtained from blood samples at 7 days by immunoprecipitation with anti-CD3 (T cells), anti-CD20 (B cells) and anti-CD14 (monocytes) antibodies. The protein content of the EVs was analyzed by data independent acquisition mass spectrometry. Differential abundance between groups was defined as fold-change ≥ 2 or ≤ 0.5 and <i>p</i> ≤ 0.05.</p> Results <p>The study enrolled 44 patients: 17 (39%) infected, 14 (82%) with poor outcomes, and 27 (61%) with no infection, 12 (44%) with poor outcomes. There were 190 proteins with differential abundance in the EVs of infected patients, 6 relevant proteins associated with poor outcome and infection: in T cell-derived EVs PSME1 (involved in apoptosis), H2B1C and MTREX (involved in transcription regulation, DNA replication and DNA repair) were more abundant; in B cell-derived EVs, COHA1 (organization of extracellular matrix) was less abundant; and in monocyte-derived EVs, PCSK9 (cholesterol metabolism) and CMC1 (energy-related metabolic pathways) were less abundant.</p> Conclusions <p>A cluster of proteins in immune system-derived EVs are involved in key biological pathways potentially linked to infection-related poor outcomes in patients with ICH.</p>

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Infection-driven proteomic signatures in immune cell–derived extracellular vesicles reflect hemorrhagic stroke outcome

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月4日

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  • Fernando Laso-García,
  • Elisa Alonso-López,
  • Dolores Piniella,
  • Exuperio Díez-Tejedor,
  • Mari Carmen Gómez-de Frutos,
  • Laura Casado-Fernández,
  • Laura Otero-Ortega,
  • Mari Paz López-Molina,
  • Rebeca Gallego-Ruiz,
  • Javier Pozo-Novoa,
  • Ángela Calzado-González,
  • Nerea Díaz-Gamero,
  • Alicia Román-San Martín,
  • Susana Bravo,
  • Rodrigo Barderas,
  • Félix Docando,
  • Blanca Fuentes,
  • Belén Juárez-Martín,
  • María Alonso de Leciñana,
  • María Gutiérrez-Fernández

摘要

Background

Analyzing the content of immune cell-derived extracellular vesicles (EVs) may reveal biomarkers that elucidate the mechanisms through which infection negatively affects outcomes in patients with intracerebral hemorrhage (ICH).

Methods

A prospective observational study in patients with acute ICH classified by the occurrence of in-hospital infection within 7 days and outcomes at 6 months, good outcome defined as an improvement of > 10 points or > 50% in NIHSS score and a mRS score 0–2. Immune cell-derived EVs were obtained from blood samples at 7 days by immunoprecipitation with anti-CD3 (T cells), anti-CD20 (B cells) and anti-CD14 (monocytes) antibodies. The protein content of the EVs was analyzed by data independent acquisition mass spectrometry. Differential abundance between groups was defined as fold-change ≥ 2 or ≤ 0.5 and p ≤ 0.05.

Results

The study enrolled 44 patients: 17 (39%) infected, 14 (82%) with poor outcomes, and 27 (61%) with no infection, 12 (44%) with poor outcomes. There were 190 proteins with differential abundance in the EVs of infected patients, 6 relevant proteins associated with poor outcome and infection: in T cell-derived EVs PSME1 (involved in apoptosis), H2B1C and MTREX (involved in transcription regulation, DNA replication and DNA repair) were more abundant; in B cell-derived EVs, COHA1 (organization of extracellular matrix) was less abundant; and in monocyte-derived EVs, PCSK9 (cholesterol metabolism) and CMC1 (energy-related metabolic pathways) were less abundant.

Conclusions

A cluster of proteins in immune system-derived EVs are involved in key biological pathways potentially linked to infection-related poor outcomes in patients with ICH.