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<p>Peripheral nerve injuries, particularly those affecting the sciatic nerve, often result in incomplete functional recovery due to the limited regenerative capacity of adult peripheral nerves. To elucidate the cellular and molecular mechanisms underlying nerve regeneration, we performed single−cell RNA sequencing (scRNA−seq) on rat sciatic nerve tissues at seven time points (Days 0, 1, 3, 5, 7, 10, and 14) following transection injury. Through unsupervised clustering, we identified four major cellular compartments—neurofibroblasts (NFs), glial cells (Glis), immune cells, and vascular cells—and delineated their dynamic trajectories during regeneration. Early responses were dominated by macrophage (Mac) and granulocyte infiltration (Day 1), followed by proliferative expansion of proliferating mesenchymal fibroblasts (NF5) and repair Schwann cells (Gli0) by Days 3–5. Vascular remodeling commenced from Day 7, while Glis progressively transitioned to mature myelinating states (Gli2/Gli5) by Day 14. Pseudotime analysis revealed subtype−specific reprogramming in both Macs and Glis, and cell–cell communication analysis uncovered key ligand–receptor interactions—particularly collagen and PTN signaling between Macs, NFs, and Glis. Bulk transcriptomic validation confirmed sustained and spatially distinct activation of the <i>TGF−</i><InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="12974_2025_3514_Article_IEq1.gif" Format="GIF" Height="17" Rendition="HTML" Resolution="72" Type="Linedraw" Width="15" /> </InlineMediaObject> <EquationSource Format="TEX">\(\:\beta\:\)</EquationSource> </InlineEquation> signaling pathway across cell types and anatomical locations. Comparative analysis with a sciatic nerve crush injury model revealed a stronger early immune response and delayed Gli recovery in transection injury, indicating a narrowed therapeutic window. Together, this work provides a time−resolved single−cell atlas of peripheral nerve regeneration, defines key regulatory circuits within the immune–NF–Gli axis, and identifies phase−specific therapeutic targets—such as early Mac heterogeneity, NF4−mediated matrix remodeling, and Schwann cell remyelination—for enhancing functional recovery following severe nerve injury.</p>

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Single−cell transcriptomic landscape of sciatic nerve after transection injury

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月23日

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  • Yiben Ouyang,
  • Mingqian Yu,
  • Tieyuan Zhang,
  • Haofeng Cheng,
  • Liang Zuo,
  • Haolin Liu,
  • Yanjun Guan,
  • Ao Liu,
  • Jiajie Chen,
  • Ruichao He,
  • Sice Wang,
  • Tianqi Su,
  • Yixiao Tan,
  • Yuhui Cu,
  • Xiaochun Zhang,
  • Xiaoyang Fu,
  • Junli Wang,
  • Jinjuan Zhao,
  • Jiang Peng,
  • Yu Wang

摘要

Peripheral nerve injuries, particularly those affecting the sciatic nerve, often result in incomplete functional recovery due to the limited regenerative capacity of adult peripheral nerves. To elucidate the cellular and molecular mechanisms underlying nerve regeneration, we performed single−cell RNA sequencing (scRNA−seq) on rat sciatic nerve tissues at seven time points (Days 0, 1, 3, 5, 7, 10, and 14) following transection injury. Through unsupervised clustering, we identified four major cellular compartments—neurofibroblasts (NFs), glial cells (Glis), immune cells, and vascular cells—and delineated their dynamic trajectories during regeneration. Early responses were dominated by macrophage (Mac) and granulocyte infiltration (Day 1), followed by proliferative expansion of proliferating mesenchymal fibroblasts (NF5) and repair Schwann cells (Gli0) by Days 3–5. Vascular remodeling commenced from Day 7, while Glis progressively transitioned to mature myelinating states (Gli2/Gli5) by Day 14. Pseudotime analysis revealed subtype−specific reprogramming in both Macs and Glis, and cell–cell communication analysis uncovered key ligand–receptor interactions—particularly collagen and PTN signaling between Macs, NFs, and Glis. Bulk transcriptomic validation confirmed sustained and spatially distinct activation of the TGF− \(\:\beta\:\) signaling pathway across cell types and anatomical locations. Comparative analysis with a sciatic nerve crush injury model revealed a stronger early immune response and delayed Gli recovery in transection injury, indicating a narrowed therapeutic window. Together, this work provides a time−resolved single−cell atlas of peripheral nerve regeneration, defines key regulatory circuits within the immune–NF–Gli axis, and identifies phase−specific therapeutic targets—such as early Mac heterogeneity, NF4−mediated matrix remodeling, and Schwann cell remyelination—for enhancing functional recovery following severe nerve injury.