Scholarlink

Background <p>The ketogenic diet (KD) has been shown to effectively reduce seizures. Ketogenic medium-chain triglycerides (MCTs) enhance brain energy metabolism in adults with neurological disorders, but their effect on peripheral metabolites in children with epilepsy is unclear. The function and mechanisms of MCTs in pediatric epilepsy remain poorly understood.</p> Methods <p>We performed untargeted LC-MS metabolomics on blood samples from children with epilepsy (<i>n</i> = 14) and healthy controls (<i>n</i> = 20), identifying octanoic acid (OA) as significantly elevated. To explore its potential role, OA was administered in a PTZ-induced seizure model in juvenile mice. Behavioral analysis, Nissl staining, and immunofluorescence demonstrated that OA modulated seizure susceptibility and activated GPR40, a receptor expressed in neurons.</p> Results <p>(1) A total of 518 metabolites were examined in plasma samples, with the top 20 metabolites selected, of which 14 were upregulated and 6 were downregulated. (2) In the PTZ-induced epilepsy model, OA significantly prolonged seizure latency by 59.86 ± 7.032&#xa0;s (<i>P</i> &lt; 0.0001), without affecting seizure grade or duration. Mechanistically, OA enhanced GPR40 activation and inhibited astrocyte activation in the hippocampus.</p> Conclusion <p>This study identified and validated peripheral blood metabolites in children with epilepsy. OA was found to exert a protective effect via GPR40 activation, enhancing neuroprotection. These findings suggest that OA may play a critical role in neuroprotection and could serve as a potential therapeutic target for pediatric epilepsy.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Screening of metabolic markers in pediatric epilepsy disorders

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月29日

查看全文
  • Yu Chen,
  • Shuo Kong,
  • Yuhui Wang,
  • Yansheng Ding,
  • Biwen Peng,
  • Jian Xu

摘要

Background

The ketogenic diet (KD) has been shown to effectively reduce seizures. Ketogenic medium-chain triglycerides (MCTs) enhance brain energy metabolism in adults with neurological disorders, but their effect on peripheral metabolites in children with epilepsy is unclear. The function and mechanisms of MCTs in pediatric epilepsy remain poorly understood.

Methods

We performed untargeted LC-MS metabolomics on blood samples from children with epilepsy (n = 14) and healthy controls (n = 20), identifying octanoic acid (OA) as significantly elevated. To explore its potential role, OA was administered in a PTZ-induced seizure model in juvenile mice. Behavioral analysis, Nissl staining, and immunofluorescence demonstrated that OA modulated seizure susceptibility and activated GPR40, a receptor expressed in neurons.

Results

(1) A total of 518 metabolites were examined in plasma samples, with the top 20 metabolites selected, of which 14 were upregulated and 6 were downregulated. (2) In the PTZ-induced epilepsy model, OA significantly prolonged seizure latency by 59.86 ± 7.032 s (P < 0.0001), without affecting seizure grade or duration. Mechanistically, OA enhanced GPR40 activation and inhibited astrocyte activation in the hippocampus.

Conclusion

This study identified and validated peripheral blood metabolites in children with epilepsy. OA was found to exert a protective effect via GPR40 activation, enhancing neuroprotection. These findings suggest that OA may play a critical role in neuroprotection and could serve as a potential therapeutic target for pediatric epilepsy.