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Background <p>Vibrational and chiroptical spectroscopy of blood plasma is an advanced experimental diagnostic approach that allows the identification of disease-specific molecular patterns. This study aimed to test its potential to distinguish between different stages of metabolic dysfunction-associated steatotic liver disease (MASLD) including liver fibrosis.</p> Methods <p>We analyzed blood plasma samples from 29 patients with metabolic dysfunction-associated steatohepatitis (MASH) and 24 MASLD patients with simple steatosis using Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy and electronic circular dichroism (ECD) spectroscopy to distinguish: (i) MASH; (ii) MASH with moderate to advanced fibrosis; and (iii) overall fibrosis.</p> Results <p>FTIR spectroscopy distinguished MASH from simple steatosis with a sensitivity of 73% and a specificity of 92%, with an area under the receiver operating characteristic curve (AUROC) of 0.92, <i>p</i> &lt; 0.001. In addition, FTIR spectroscopy identified MASH with moderate to advanced fibrosis (F2–3) from MASH/steatosis with no to mild fibrosis (F0–1) with a sensitivity of 87% and specificity of 78% (AUROC 0.91, <i>p</i> &lt; 0.001). Furthermore, regardless of the MASH/steatosis diagnosis, fibrosis (F1–3) was distinguished from F0 by the combination of data from the three spectroscopic methods with a sensitivity of 79% and a specificity of 88% (AUROC 0.91, <i>p</i> &lt; 0.001). Both FTIR and Raman spectroscopy alone discriminated all target groups with an AUROC &gt; 0.82.</p> Conclusions <p>This pilot study revealed the potential of blood plasma spectroscopy to identify and differentiate patients with various stages of MASLD.</p>

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Molecular spectroscopy of blood plasma differentiates metabolic dysfunction-associated steatohepatitis from steatosis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月6日

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  • Barbora Nováková,
  • Ondřej Vrtělka,
  • Kateřina Králová,
  • Lucie Habartová,
  • Václav Šmíd,
  • Karel Dvořák,
  • Jaromír Petrtýl,
  • Kateřina Žížalová,
  • Jan Stříteský,
  • Libor Vítek,
  • Martin Leníček,
  • Vladimír Setnička,
  • Radan Brůha

摘要

Background

Vibrational and chiroptical spectroscopy of blood plasma is an advanced experimental diagnostic approach that allows the identification of disease-specific molecular patterns. This study aimed to test its potential to distinguish between different stages of metabolic dysfunction-associated steatotic liver disease (MASLD) including liver fibrosis.

Methods

We analyzed blood plasma samples from 29 patients with metabolic dysfunction-associated steatohepatitis (MASH) and 24 MASLD patients with simple steatosis using Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy and electronic circular dichroism (ECD) spectroscopy to distinguish: (i) MASH; (ii) MASH with moderate to advanced fibrosis; and (iii) overall fibrosis.

Results

FTIR spectroscopy distinguished MASH from simple steatosis with a sensitivity of 73% and a specificity of 92%, with an area under the receiver operating characteristic curve (AUROC) of 0.92, p < 0.001. In addition, FTIR spectroscopy identified MASH with moderate to advanced fibrosis (F2–3) from MASH/steatosis with no to mild fibrosis (F0–1) with a sensitivity of 87% and specificity of 78% (AUROC 0.91, p < 0.001). Furthermore, regardless of the MASH/steatosis diagnosis, fibrosis (F1–3) was distinguished from F0 by the combination of data from the three spectroscopic methods with a sensitivity of 79% and a specificity of 88% (AUROC 0.91, p < 0.001). Both FTIR and Raman spectroscopy alone discriminated all target groups with an AUROC > 0.82.

Conclusions

This pilot study revealed the potential of blood plasma spectroscopy to identify and differentiate patients with various stages of MASLD.