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Background <p>Melanoma is a highly aggressive form of skin cancer. Despite significant advances in targeted therapies and immunotherapeutic approaches, some patients still have poor response rates, making a deeper understanding of melanoma pathogenesis essential.</p> Methods <p>The expression of Claspin (CLSPN), prognosis and immune infiltration in skin cutaneous melanoma patients were analyzed by public databases. Immunohistochemistry was used to validate. Moreover, quantitative real-time polymerase chain reaction analysis, western blot, cell counting kit-8 assay, colony formation assay, flow cytometry, animal experiments, and RNA-seq were applied to explore its biological functions and potential molecular mechanisms of CLSPN in melanoma.</p> Results <p>Our results demonstrated that abnormal CLSPN expression was correlated with poor prognosis in melanoma. Meanwhile, CLSPN may promote melanoma growth and progression in <i>vivo</i> and in <i>vitro</i> through IFI44L/JAK/STAT1 signaling. Additionally, CLSPN was associated with negative immune microenvironment in melanoma and may be related to polarization of tumor associated macrophages towards M2-type.</p> Conclusions <p>These findings suggest that CLSPN may be a promising new target for melanoma and accelerate personalized therapeutic strategies.</p> Graphical abstract <p></p>

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Potential of CLSPN as a therapeutic target in melanoma: a key player in melanoma progression and tumor microenvironment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月24日

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  • Yongyi Xie,
  • Ruoqi Wang,
  • Mingyuan Xu,
  • Jiashe Chen,
  • Wei Tan,
  • Yanbin Chen,
  • Yun Bai,
  • Nanhui Wu,
  • Fei Wu,
  • Xiaoxiang Xu,
  • Xin Ma,
  • Yeqiang Liu

摘要

Background

Melanoma is a highly aggressive form of skin cancer. Despite significant advances in targeted therapies and immunotherapeutic approaches, some patients still have poor response rates, making a deeper understanding of melanoma pathogenesis essential.

Methods

The expression of Claspin (CLSPN), prognosis and immune infiltration in skin cutaneous melanoma patients were analyzed by public databases. Immunohistochemistry was used to validate. Moreover, quantitative real-time polymerase chain reaction analysis, western blot, cell counting kit-8 assay, colony formation assay, flow cytometry, animal experiments, and RNA-seq were applied to explore its biological functions and potential molecular mechanisms of CLSPN in melanoma.

Results

Our results demonstrated that abnormal CLSPN expression was correlated with poor prognosis in melanoma. Meanwhile, CLSPN may promote melanoma growth and progression in vivo and in vitro through IFI44L/JAK/STAT1 signaling. Additionally, CLSPN was associated with negative immune microenvironment in melanoma and may be related to polarization of tumor associated macrophages towards M2-type.

Conclusions

These findings suggest that CLSPN may be a promising new target for melanoma and accelerate personalized therapeutic strategies.

Graphical abstract