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<p>During mammal meiosis, proper cytokinesis is essential for oocyte maturation. This process depends on actin cap formation and cortical polarity establishment. In this study, we reported that DEP domain-containing protein 7 (DEPDC7) functioned as a regulator of oocyte cytokinesis. Conditional knockout of DEPDC7 in oocytes resulted in triploid zygotes, impaired early embryo development, and ultimately led to female subfertility. Chromosome spread analysis showed that superovulated oocytes lacking DEPDC7 were diploid. Although the assembly and migration of the spindles were normal in DEPDC7-deleted oocytes during meiosis I, actin cap assembly and first polar body extrusion were severely disrupted. Furthermore, we found that DEPDC7 colocalized and interacted with ARP2/3 complex, regulating its subcortical enrichment. Taken together, our findings reveal the function of DEPDC7 is required for polarity establishment and coupling chromosome segregation to cytokinesis during mouse oocyte meiosis.</p>

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DEPDC7 couples homologous chromosome segregation to asymmetric cytokinesis through recruiting ARP2/3 complex in oocyte meiosis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月14日

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  • Jia-Ni Guo,
  • Si-Min Sun,
  • Xue-Mei Yang,
  • Chao Jiang,
  • Yi-Ke Lu,
  • Yuan-Hong Xu,
  • Bing-Wang Zhao,
  • Zhen-Bo Wang

摘要

During mammal meiosis, proper cytokinesis is essential for oocyte maturation. This process depends on actin cap formation and cortical polarity establishment. In this study, we reported that DEP domain-containing protein 7 (DEPDC7) functioned as a regulator of oocyte cytokinesis. Conditional knockout of DEPDC7 in oocytes resulted in triploid zygotes, impaired early embryo development, and ultimately led to female subfertility. Chromosome spread analysis showed that superovulated oocytes lacking DEPDC7 were diploid. Although the assembly and migration of the spindles were normal in DEPDC7-deleted oocytes during meiosis I, actin cap assembly and first polar body extrusion were severely disrupted. Furthermore, we found that DEPDC7 colocalized and interacted with ARP2/3 complex, regulating its subcortical enrichment. Taken together, our findings reveal the function of DEPDC7 is required for polarity establishment and coupling chromosome segregation to cytokinesis during mouse oocyte meiosis.