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Background <p>Cervical cancer (CC) remains a significant health concern for women worldwide, with poor prognosis often linked to late diagnosis. This study aimed to explore the clinical significance and molecular mechanisms of long non-coding RNA BZRAP1-AS1.</p> Methods <p>A total of 105 CC patients were enrolled, and paired tumor and normal tissues were collected. The expression levels of BZRAP1-AS1 and miR-541-3p were quantified by qRT-PCR. The direct binding between BZRAP1-AS1 and miR-541-3p was validated using a dual-luciferase reporter assay. Functional assays, including CCK-8, Transwell, and flow cytometry analysis, were performed in CC cell lines.</p> Results <p>BZRAP1-AS1 was upregulated in CC tissues and correlated with advanced FIGO stage and lymph node metastasis (<i>P</i> &lt; 0.05). Kaplan–Meier analysis revealed that patients with high BZRAP1‑AS1 expression had significantly shorter overall survival (log‑rank <i>P</i> = 0.007). Multivariate Cox regression confirmed high BZRAP1‑AS1 expression as an independent predictor of poor prognosis (HR = 3.031, 95% CI = 1.136–8.090). Silencing of BZRAP1-AS1 inhibited cell proliferation and invasion, while promoting apoptosis. Mechanistically, BZRAP1-AS1 acted as a competing endogenous RNA (ceRNA) to sponge miR-541-3p. Rescue experiments indicated that inhibition of miR-541-3p reversed the tumor-suppressive effects resulting from BZRAP1-AS1 knockdown.</p> Conclusion <p>Our findings suggest that BZRAP1-AS1 may serve as an independent prognostic marker in cervical cancer. Mechanistically, it appears to promote tumor progression by downregulating miR‑541‑3p. The BZRAP1‑AS1/miR‑541‑3p axis thus warrants further investigation, though its translational potential requires validation through larger multi‑center studies.</p>

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Effect of immune-related lncRNA BZRAP1-AS1/miR-541-3p and its molecular mechanism in cervical cancer

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月9日

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  • Zhixiang Zou,
  • Lina Huang,
  • Ke Yin,
  • Yi Li,
  • Mengying Tang,
  • Chao He

摘要

Background

Cervical cancer (CC) remains a significant health concern for women worldwide, with poor prognosis often linked to late diagnosis. This study aimed to explore the clinical significance and molecular mechanisms of long non-coding RNA BZRAP1-AS1.

Methods

A total of 105 CC patients were enrolled, and paired tumor and normal tissues were collected. The expression levels of BZRAP1-AS1 and miR-541-3p were quantified by qRT-PCR. The direct binding between BZRAP1-AS1 and miR-541-3p was validated using a dual-luciferase reporter assay. Functional assays, including CCK-8, Transwell, and flow cytometry analysis, were performed in CC cell lines.

Results

BZRAP1-AS1 was upregulated in CC tissues and correlated with advanced FIGO stage and lymph node metastasis (P < 0.05). Kaplan–Meier analysis revealed that patients with high BZRAP1‑AS1 expression had significantly shorter overall survival (log‑rank P = 0.007). Multivariate Cox regression confirmed high BZRAP1‑AS1 expression as an independent predictor of poor prognosis (HR = 3.031, 95% CI = 1.136–8.090). Silencing of BZRAP1-AS1 inhibited cell proliferation and invasion, while promoting apoptosis. Mechanistically, BZRAP1-AS1 acted as a competing endogenous RNA (ceRNA) to sponge miR-541-3p. Rescue experiments indicated that inhibition of miR-541-3p reversed the tumor-suppressive effects resulting from BZRAP1-AS1 knockdown.

Conclusion

Our findings suggest that BZRAP1-AS1 may serve as an independent prognostic marker in cervical cancer. Mechanistically, it appears to promote tumor progression by downregulating miR‑541‑3p. The BZRAP1‑AS1/miR‑541‑3p axis thus warrants further investigation, though its translational potential requires validation through larger multi‑center studies.