Scholarlink

<p>The pathological progression of dry eye disease (DED) involves a vicious cycle of oxidative stress and inflammation, posing a critical therapeutic challenge. Conventional therapies, such as cyclosporine A (CsA), are limited by poor corneal permeability and low ocular bioavailability. Here, we developed a novel, biocompatible nano-eye drop formulation using <i>Chrysanthemum indicum</i> L.-derived extracellular vesicles (CELNs) as a natural nanocarrier to engineer CsA-loaded CELNs (CsA@CELNs) for synergistic DED therapy. By combining CsA-mediated immunoregulation with enhanced corneal permeability and the intrinsic antioxidant and anti-inflammatory activities of CELN, the CsA@CELNs effectively disrupts the core pathogenic feedback loop of DED. In vitro and in vivo data demonstrated that a one-week, twice daily topical treatment with CsA@CELNs alleviated oxidative stress by scavenging reactive oxygen species (ROS) and activating the Nrf2/HO-1/NQO1 signaling pathway, while concurrently suppressing inflammation through inhibiting the NF-κB pathway and promoting macrophage repolarization from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype. In a murine DED model, CsA@CELNs eye drop significantly restored tear secretion, promoted regeneration of corneal and conjunctival cells, and improved lacrimal gland histology. This multi-targeting CsA@CELNs system not only provides a safe and effective nanotherapeutic strategy for DED but also establishes plant-derived extracellular vesicles as a promising drug delivery platform for treating ocular surface and other inflammatory diseases.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Chrysanthemum indicum L.-derived extracellular vesicles enhance the therapeutic efficacy of cyclosporine a against dry eye disease

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月29日

查看全文
  • Jing Li,
  • Pei Xin Lee,
  • Chengkai Shi,
  • Dengxuan Mao,
  • Xiumei Liu,
  • Zi Yan,
  • Hong Zou,
  • Ming Cai,
  • Zimei Wu,
  • Yaqi Lyu,
  • Nianping Feng

摘要

The pathological progression of dry eye disease (DED) involves a vicious cycle of oxidative stress and inflammation, posing a critical therapeutic challenge. Conventional therapies, such as cyclosporine A (CsA), are limited by poor corneal permeability and low ocular bioavailability. Here, we developed a novel, biocompatible nano-eye drop formulation using Chrysanthemum indicum L.-derived extracellular vesicles (CELNs) as a natural nanocarrier to engineer CsA-loaded CELNs (CsA@CELNs) for synergistic DED therapy. By combining CsA-mediated immunoregulation with enhanced corneal permeability and the intrinsic antioxidant and anti-inflammatory activities of CELN, the CsA@CELNs effectively disrupts the core pathogenic feedback loop of DED. In vitro and in vivo data demonstrated that a one-week, twice daily topical treatment with CsA@CELNs alleviated oxidative stress by scavenging reactive oxygen species (ROS) and activating the Nrf2/HO-1/NQO1 signaling pathway, while concurrently suppressing inflammation through inhibiting the NF-κB pathway and promoting macrophage repolarization from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype. In a murine DED model, CsA@CELNs eye drop significantly restored tear secretion, promoted regeneration of corneal and conjunctival cells, and improved lacrimal gland histology. This multi-targeting CsA@CELNs system not only provides a safe and effective nanotherapeutic strategy for DED but also establishes plant-derived extracellular vesicles as a promising drug delivery platform for treating ocular surface and other inflammatory diseases.

Graphical Abstract