Scholarlink

<p>Protein-based nanoparticles hold great promise for bioactive molecule delivery, but conventional fabrication routes are often complex and rely on surfactants or organic solvents. Here, we reported a crosslinker-free strategy to engineer α-lactalbumin (ALA) nanoparticles via Ca²⁺ mediated ionic bridging and electrospraying, enabling efficient encapsulation of acidic fibroblast growth factor (aFGF). These aFGF@ALA nanoparticles were subsequently integrated into electrospun poly(vinyl alcohol) (PVA) nanofiber matrices to construct hybrid dressings (aFGF@ALA NPs/PVA ENMs). The resulting nanostructures combine the structural support of nanofibers with the controlled release capacity of protein nanoparticles, yielding a synergistic platform for therapeutic delivery. In vitro, the hybrid dressings promoted fibroblasts and keratinocytes proliferation and migration with excellent cytocompatibility. In vivo, they accelerated burn wound repair by enhancing re-epithelialization, collagen I/III remodeling, and angiogenesis. This work introduces a facile and generalizable strategy for designing functional protein-based nanoparticles and demonstrates their integration with nanofiber scaffolds as a versatile platform for growth factor delivery and tissue regeneration.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ionic-bridge engineered α-lactalbumin nanoparticles integrated into electrospun nanofibers for controlled growth factor delivery and burn wound repair

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年4月27日

查看全文
  • Liangwei Si,
  • Peixin Liu,
  • Yasir Faraz Abbasi,
  • Yang Chen,
  • Xueying Xu,
  • Jiahui Li,
  • Xiaoxuan Zhu,
  • Yuxin Li,
  • Vito Foderà,
  • Dongmei Cun,
  • Xiong Guo,
  • Mingshi Yang

摘要

Protein-based nanoparticles hold great promise for bioactive molecule delivery, but conventional fabrication routes are often complex and rely on surfactants or organic solvents. Here, we reported a crosslinker-free strategy to engineer α-lactalbumin (ALA) nanoparticles via Ca²⁺ mediated ionic bridging and electrospraying, enabling efficient encapsulation of acidic fibroblast growth factor (aFGF). These aFGF@ALA nanoparticles were subsequently integrated into electrospun poly(vinyl alcohol) (PVA) nanofiber matrices to construct hybrid dressings (aFGF@ALA NPs/PVA ENMs). The resulting nanostructures combine the structural support of nanofibers with the controlled release capacity of protein nanoparticles, yielding a synergistic platform for therapeutic delivery. In vitro, the hybrid dressings promoted fibroblasts and keratinocytes proliferation and migration with excellent cytocompatibility. In vivo, they accelerated burn wound repair by enhancing re-epithelialization, collagen I/III remodeling, and angiogenesis. This work introduces a facile and generalizable strategy for designing functional protein-based nanoparticles and demonstrates their integration with nanofiber scaffolds as a versatile platform for growth factor delivery and tissue regeneration.

Graphical Abstract