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<p>A novel dual-quenching electrochemiluminescence (ECL) immunosensor based on ECL resonance energy transfer (ECL-RET) strategy was developed for sensitive detection of soluble suppression of tumorigenicity 2 (sST2) protein. The sensor developed a self-accelerated ECL emitter composed of Au-decorated graphitic carbon nitride (g-C<sub>3</sub>N<sub>4</sub>) nanosheets and Materials of Institute Lavoisier-100(Fe) (MIL-100(Fe)). In this system, the reversible Fe<sup>2+</sup>/Fe<sup>3+</sup> redox cycling in MIL-100(Fe) markedly enhanced the ECL emission from the g-C<sub>3</sub>N<sub>4</sub>/S<sub>2</sub>O<sub>8</sub><sup>2−</sup> pair by promoting the generation of sulfate radical. Simultaneously, the incorporated Au nanoparticles facilitated electron transfer and provided abundant sites for biomolecular immobilization, collectively establishing a robust “signal-on” state. Signal quenching was achieved using Pt@CuS nanoflowers as synergistic ECL quenchers. Their high antibody-loading capacity and efficient ECL-RET capability acted in concert to drastically reduce the signal, thereby inducing a pronounced “signal-off” state. The optimized “on–off” ECL immunosensor demonstrated a satisfactory linear range (0.001–100&#xa0;ng&#xa0;mL<sup>−1</sup>) and an ultralow detection limit of 0.547&#xa0;pg&#xa0;mL<sup>−1</sup> for sST2. This work established a reliable platform for sST2 quantification, holding substantial potential for advancing heart failure research and clinical early diagnosis.</p> Graphical Abstract <p></p>

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Au-g-C3N4@MIL-100/Pt@CuS-induced self-accelerated dual-quenching ECL-RET strategy for sensitive sST2 detection

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月12日

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  • Xinrui He,
  • Zhujun Ai,
  • Diao Yu,
  • Xiang Yu,
  • Shijia Ding,
  • Ling Zeng,
  • Wei Yang,
  • Qinggang Zhang

摘要

A novel dual-quenching electrochemiluminescence (ECL) immunosensor based on ECL resonance energy transfer (ECL-RET) strategy was developed for sensitive detection of soluble suppression of tumorigenicity 2 (sST2) protein. The sensor developed a self-accelerated ECL emitter composed of Au-decorated graphitic carbon nitride (g-C3N4) nanosheets and Materials of Institute Lavoisier-100(Fe) (MIL-100(Fe)). In this system, the reversible Fe2+/Fe3+ redox cycling in MIL-100(Fe) markedly enhanced the ECL emission from the g-C3N4/S2O82− pair by promoting the generation of sulfate radical. Simultaneously, the incorporated Au nanoparticles facilitated electron transfer and provided abundant sites for biomolecular immobilization, collectively establishing a robust “signal-on” state. Signal quenching was achieved using Pt@CuS nanoflowers as synergistic ECL quenchers. Their high antibody-loading capacity and efficient ECL-RET capability acted in concert to drastically reduce the signal, thereby inducing a pronounced “signal-off” state. The optimized “on–off” ECL immunosensor demonstrated a satisfactory linear range (0.001–100 ng mL−1) and an ultralow detection limit of 0.547 pg mL−1 for sST2. This work established a reliable platform for sST2 quantification, holding substantial potential for advancing heart failure research and clinical early diagnosis.

Graphical Abstract