<p>Uncontrolled acute and persistent bleeding, as well as with infection, is a great challenge because of the high mortality during treating the patients with injuries, complex surgery or bone marrow failure. Here, we develop an external form of natural components which is based on phosphorylated methacrylated gelatin (GelMA, G) cryogel (GP) loaded with tannic acid (TA)-mixed copper ion (Cu²⁺) mesoporous bioactive glasses (MBG), named after GP@MBG-Cu-TA cryogel, to address the goals of reduce persistent bleeding and enhance antibacterial activity. Structurally, GP@MBG-Cu-TA cryogel is based on GP, MBG loaded with TA and Cu²⁺ adheres to GP via hydrogen bonding. In vitro, GP@MBG-Cu-TA cryogel displays a good biocompatibility, hemostatic and antimicrobial capability. In vivo studies, GP@MBG-Cu-TA cryogel can enhance the hemostatic effect in the liver injury in SD rats for the acute bleeding, as well as in the aplastic anemia and hemophilia A mice with tail amputation for the persistent bleeding. In addition, GP@MBG-Cu-TA cryogel accelerates the skin wound repair in the mice with the bacterial contamination at the injury site. In sum, GP@MBG-Cu-TA cryogel is not only endowed with dual function of hemostatic and antimicrobial capability, but also can stop bleeding of the objects with either normal or abnormal coagulation function. Thus, GP@MBG-Cu-TA cryogel provides a promising candidate dressing for managing bleeding and bacterial complications in clinic.</p> Graphical abstract <p></p>