Scholarlink

Objective <p>To develop and evaluate a quercetin-loaded silicon dioxide–graphene nanocomposite (Que@SiO₂-GNPs) for targeted macrophage modulation and epithelial protection in a Mycoplasma-induced pneumonia model.</p> Methods <p>Que@SiO₂-GNPs were synthesized via graphene oxide dispersion, sol–gel silica coating, and quercetin adsorption. Physicochemical properties were characterized by XPS, XRD, and FTIR. The therapeutic efficacy was evaluated in MP-infected rat models via histopathology, ELISA, flow cytometry, immunostaining, and Western blot. Cellular uptake, polarization, and functional assays were performed in primary AMs and BEAS-2B epithelial cells.</p> Results <p>Que@SiO₂-GNPs exhibited successful surface modification and quercetin incorporation with stable physicochemical properties. In vivo imaging demonstrated pulmonary targeting and favorable biocompatibility. In MP-infected rats, Que@SiO₂-GNPs significantly reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), alleviated lung injury, and promoted tissue repair. Mechanistically, the nanocomposites downregulated iNOS and CD86 while enhancing CD206 expression in AMs, indicating M2 polarization. Conditioned AMs improved epithelial proliferation, reduced oxidative stress and apoptosis, and upregulated SPLUNC1 expression. These outcomes were validated by EdU, ROS, Annexin V/PI, LDH assays, and protein expression analyses.</p> Conclusions <p>Que@SiO₂-GNPs effectively reprogrammed macrophage phenotypes toward M2, suppressed inflammation, and conferred epithelial protection in Mycoplasma-induced pneumonia. This nanoplatform represents a promising immunomodulatory strategy for pulmonary infectious diseases.</p> Clinical trial number <p>Not applicable.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Quercetin-loaded silicon dioxide–graphene nanoparticles promotes M2 macrophage reprogramming in mycoplasma-induced pneumonia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月19日

查看全文
  • Xiuxiu Liu,
  • Xiufeng Chen,
  • Yonghong Jiang,
  • Wen Li,
  • Xiaoqin Fei,
  • Xiao Li,
  • Zhiyan Jiang

摘要

Objective

To develop and evaluate a quercetin-loaded silicon dioxide–graphene nanocomposite (Que@SiO₂-GNPs) for targeted macrophage modulation and epithelial protection in a Mycoplasma-induced pneumonia model.

Methods

Que@SiO₂-GNPs were synthesized via graphene oxide dispersion, sol–gel silica coating, and quercetin adsorption. Physicochemical properties were characterized by XPS, XRD, and FTIR. The therapeutic efficacy was evaluated in MP-infected rat models via histopathology, ELISA, flow cytometry, immunostaining, and Western blot. Cellular uptake, polarization, and functional assays were performed in primary AMs and BEAS-2B epithelial cells.

Results

Que@SiO₂-GNPs exhibited successful surface modification and quercetin incorporation with stable physicochemical properties. In vivo imaging demonstrated pulmonary targeting and favorable biocompatibility. In MP-infected rats, Que@SiO₂-GNPs significantly reduced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), alleviated lung injury, and promoted tissue repair. Mechanistically, the nanocomposites downregulated iNOS and CD86 while enhancing CD206 expression in AMs, indicating M2 polarization. Conditioned AMs improved epithelial proliferation, reduced oxidative stress and apoptosis, and upregulated SPLUNC1 expression. These outcomes were validated by EdU, ROS, Annexin V/PI, LDH assays, and protein expression analyses.

Conclusions

Que@SiO₂-GNPs effectively reprogrammed macrophage phenotypes toward M2, suppressed inflammation, and conferred epithelial protection in Mycoplasma-induced pneumonia. This nanoplatform represents a promising immunomodulatory strategy for pulmonary infectious diseases.

Clinical trial number

Not applicable.