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<p>The <i>mcr-9</i> allele is typically located on IncHI2 type plasmids, but there are only few reports describing the co-occurrence of the <i>bla</i><sub>NDM</sub> gene on the same plasmid. Our aims were to describe the spread of an IncHI2A plasmid harboring both the <i>mcr-9</i> and the <i>bla</i><sub>NDM-1</sub> genes in a multicenter study in Israel. All New-Delhi Metallo-β-lactamase-producing Enterobacterales (NDME) isolated from three medical centres in Israel between January 2018 and July 2019 were included. The <i>mcr-9.1</i> gene was identified in 37/212 (17.4%) of the isolates, mostly in <i>Enterobacter cloacae</i> (34/37, 92%). The <i>mcr-9.1</i> gene was also identified in <i>Klebsiella pneumoniae</i> sequence types (ST)-76 (<i>n</i> = 2) and <i>Escherichia coli</i> ST-69 (<i>n</i> = 1). In one hospital, out of 32 <i>E. cloacae</i> isolates, 19 (51.35%) were clustered into five transmission clusters of the ST-511, ST-1261 and ST-1750. Four subtypes of a ~ 290&#xa0;kb IncHI2A type plasmid were identified in all isolates that co-harbored the <i>mcr-9.1</i> and the <i>bla</i><sub>NDM-1</sub> genes. This plasmid was identified in all isolates, with four sub-communities (sc), with sc-4 identified in all three species. The resistance genes were surrounded by the IS<i>26</i> (<i>mcr-9.1</i>) or by the IS<i>Aba125</i> and the IS<i>300</i> (<i>bla</i><sub>NDM-1</sub>) mobile elements. The dissemination of the <i>mcr-9.1</i> and the <i>bla</i><sub>NDM-1</sub> genes was accelerated via clonal spread and the dual carriage on a single plasmid.</p>

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Dissemination of a IncHI2A plasmid co-harboring the mcr-9 and blaNDM-1 genes in Israeli hospitals

  • 期刊论文

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  • 发表日期：

    2025年8月20日

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  • Amos Adler,
  • Stefany Ayala-Montaño,
  • Mark V. Assous,
  • Yuval Geffen,
  • Sandra Reuter

摘要

The mcr-9 allele is typically located on IncHI2 type plasmids, but there are only few reports describing the co-occurrence of the blaNDM gene on the same plasmid. Our aims were to describe the spread of an IncHI2A plasmid harboring both the mcr-9 and the blaNDM-1 genes in a multicenter study in Israel. All New-Delhi Metallo-β-lactamase-producing Enterobacterales (NDME) isolated from three medical centres in Israel between January 2018 and July 2019 were included. The mcr-9.1 gene was identified in 37/212 (17.4%) of the isolates, mostly in Enterobacter cloacae (34/37, 92%). The mcr-9.1 gene was also identified in Klebsiella pneumoniae sequence types (ST)-76 (n = 2) and Escherichia coli ST-69 (n = 1). In one hospital, out of 32 E. cloacae isolates, 19 (51.35%) were clustered into five transmission clusters of the ST-511, ST-1261 and ST-1750. Four subtypes of a ~ 290 kb IncHI2A type plasmid were identified in all isolates that co-harbored the mcr-9.1 and the blaNDM-1 genes. This plasmid was identified in all isolates, with four sub-communities (sc), with sc-4 identified in all three species. The resistance genes were surrounded by the IS26 (mcr-9.1) or by the ISAba125 and the IS300 (blaNDM-1) mobile elements. The dissemination of the mcr-9.1 and the blaNDM-1 genes was accelerated via clonal spread and the dual carriage on a single plasmid.