<p><i>Zymomonas mobilis</i> is an ethanologenic Alphaproteobacterium with many interesting characteristics for fundamental research and applied microbial engineering. Although genetic engineering has been established for <i>Z. mobilis</i> since the 1980s, a rich set of inducible transcriptional regulators is still unavailable. In this work, seven different chemically inducible promoters have been systematically tested for their functionality in <i>Z. mobilis</i>. In particular, for the first time, NahR-P_salTTC, VanR^AM-P_vanCC, CinR^AM-P_cin and LuxR-P_luxB have been characterized in <i>Z. mobilis</i>, alongside the commonly used regulator-promoter pairs TetR-P_tet and LacI-P_{lacT7A1_O3O4}, and the less commonly used XylS-P_m. All promoters investigated in this work are compatible with the Golden Gate modular cloning framework Zymo-Parts. Characterization was carried out with a shuttle vector backbone based on pZMO7, which has so far been rarely used for applications in <i>Z. mobilis</i> but is stable without selection and generates high and uniform levels of expression. From the experimental results presented, it can be concluded that VanR^AM-P_vanCC and CinR^AM-P_cin are particularly promising for broad use in the <i>Z. mobilis</i> community.</p> Graphical Abstract <p></p>