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Background <p>Used motor oil (UMO) is a dangerous environmental pollutant that needs to be treated effectively. This work introduces a novel approach for producing bioelectricity and UMO biodegradation simultaneously in a single-chamber microbial fuel cell (SCMFC) using native mixed bacterial cultures.</p> Results <p>Under certain conditions (2% oil, 1% peptone, 4% inoculum, 21 days), the optimized bacterial culture degraded UMO by about 80%. Through bioelectrochemical studies, a maximum voltage of 257 mV and a power density of 36.6 mW/m² were demonstrated, showing a strong correlation between UMO removal and electricity generation. Moreover, metagenomic data showed that Firmicutes, particularly <i>Bacillus</i>, dominated the biofilm at roughly 65%. Fourier Transform Infrared (FTIR) and Gas Chromatography-Mass Spectroscopy (GC-MS) verified the breakdown of complex hydrocarbon molecules, highlighting their crucial role in UMO biodegradation and bioenergy production. The effective elimination of UMOs and simultaneous power generation, supported by metagenomic and biochemical tests, showed the microbial activity and hydrocarbon breakdown.</p> Conclusions <p>The results suggest SCMFC technology as a sustainable solution for managing petroleum waste while producing renewable energy.</p>

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Biodegradation potential of used motor oil by mixed bacterial community: optimization, emulsification activity, bioelectrochemical and metagenomics analyses using single chamber microbial fuel cell

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年12月28日

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  • Ebtehag A. E. Sakr,
  • Nahla M. Mansour,
  • Hanaa M. Sabaa,
  • K. M. El-khatib,
  • Dena Z. Khater

摘要

Background

Used motor oil (UMO) is a dangerous environmental pollutant that needs to be treated effectively. This work introduces a novel approach for producing bioelectricity and UMO biodegradation simultaneously in a single-chamber microbial fuel cell (SCMFC) using native mixed bacterial cultures.

Results

Under certain conditions (2% oil, 1% peptone, 4% inoculum, 21 days), the optimized bacterial culture degraded UMO by about 80%. Through bioelectrochemical studies, a maximum voltage of 257 mV and a power density of 36.6 mW/m² were demonstrated, showing a strong correlation between UMO removal and electricity generation. Moreover, metagenomic data showed that Firmicutes, particularly Bacillus, dominated the biofilm at roughly 65%. Fourier Transform Infrared (FTIR) and Gas Chromatography-Mass Spectroscopy (GC-MS) verified the breakdown of complex hydrocarbon molecules, highlighting their crucial role in UMO biodegradation and bioenergy production. The effective elimination of UMOs and simultaneous power generation, supported by metagenomic and biochemical tests, showed the microbial activity and hydrocarbon breakdown.

Conclusions

The results suggest SCMFC technology as a sustainable solution for managing petroleum waste while producing renewable energy.