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<p>Bacillomycin L, a cyclic lipopeptide derived from <i>Bacillus amyloliquefaciens</i>, has great potential for developing biochemical phytofungicides. In this study, a mutant M86 with high anti-<i>Botrytis cinerea</i> activity was obtained by atmospheric and room-temperature plasma (ARTP) mutagenesis from the original strain 1841, with bacillomycin L yield increasing from 244.22 to 415.89&#xa0;mg/L. Mass spectrometry analysis identified that the main active compounds were C14-, C15-, and C16-bacillomycin L. Re-sequencing M86 showed that ARTP mutagenesis resulted in the effective mutations in sigma factors and ABC transporter proteins. Transcriptome sequencing further revealed the significant up-regulation of the <i>bmyDABC</i> and <i>ytrBCC2DEF</i> gene cluster involved in bacillomycin L synthesis and transporter, respectively, in M86. Bacillomycin L yield was further boosted to 676.47&#xa0;mg/L after optimizing the fermentation medium by adjusting glycine, serine, and K<sub>2</sub>HPO<sub>4</sub> concentrations. Bacillomycin L exhibited broad inhibitory activity against 17 fungi and nine bacterial species. This investigation provides a foundation for bacillomycin L production and application.</p>

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Improving the bacillomycin L production in Bacillus amyloliquefaciens by atmospheric and room-temperature plasma combined with Box-Behnken design

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月23日

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  • Andong Zhang,
  • Bin Yang,
  • Yudong Ma,
  • Ruolin Li,
  • Zhiwei Zhou,
  • Chen Luo,
  • Yiyang Zhang,
  • Yuhan Zhang,
  • Ya Dan,
  • Qun Sun

摘要

Bacillomycin L, a cyclic lipopeptide derived from Bacillus amyloliquefaciens, has great potential for developing biochemical phytofungicides. In this study, a mutant M86 with high anti-Botrytis cinerea activity was obtained by atmospheric and room-temperature plasma (ARTP) mutagenesis from the original strain 1841, with bacillomycin L yield increasing from 244.22 to 415.89 mg/L. Mass spectrometry analysis identified that the main active compounds were C14-, C15-, and C16-bacillomycin L. Re-sequencing M86 showed that ARTP mutagenesis resulted in the effective mutations in sigma factors and ABC transporter proteins. Transcriptome sequencing further revealed the significant up-regulation of the bmyDABC and ytrBCC2DEF gene cluster involved in bacillomycin L synthesis and transporter, respectively, in M86. Bacillomycin L yield was further boosted to 676.47 mg/L after optimizing the fermentation medium by adjusting glycine, serine, and K2HPO4 concentrations. Bacillomycin L exhibited broad inhibitory activity against 17 fungi and nine bacterial species. This investigation provides a foundation for bacillomycin L production and application.