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<p>Rice blast, a disease caused by <i>Magnaporthe oryzae</i>, significantly threatens global rice production. To improve the anti-<i>M. oryzae</i> activity of <i>Streptomyces bikiniensis</i> HD-087 metabolites, the effects of inducer, <i>Magnaporthe oryzae</i> acellular filtrate, on secondary metabolism of <i>S. bikiniensis</i> HD-087 were studied. The results showed that <i>M. oryzae</i> cell-free filtrate cultured for 96 h served as the most effective inducer, significantly enhancing the anti-<i>M. oryzae</i> activity of metabolites of <i>S. bikiniensis</i> HD-087 and increasing the diameter of the inhibitory zone by 2.96 mm. The inhibition rates of <i>M. oryzae</i> colony diameter and spore germination in the induced group were 12.39% and 39.6% higher than those in the non-induced group, respectively. Metabolomic profiling of strain HD-087 highlighted substantial differences between the induced and non-induced groups. At 48 h of fermentation, a total of 705 distinct metabolites were identified, while at 96 h this number decreased to 321. Moreover, induction markedly altered primary pathways such as the tricarboxylic acid cycle, amino acid biosynthesis, and fatty acid metabolism in <i>S. bikiniensis</i> HD-087. qPCR analysis showed that <i>nrps</i> genes and <i>pks</i> genes in the induced group were significantly up-regulated by 9.92 ± 0.51 and 2.71 ± 0.17 times, respectively, and biotin carboxylase activity was also increased 26.63%. These results provide a theoretical basis for using inducers to enhance the antimicrobial ability of <i>Streptomyces</i>.</p> Graphical Abstract <p></p>

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Effects of Magnaporthe oryzae cell-free filtrate on the secondary metabolism of Streptomyces bikiniensis HD-087: a non-targeted metabolomics analysis

  • 期刊论文

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  • 发表日期：

    2025年4月21日

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  • Jiahan Gang,
  • Qingqing Tian,
  • Chunmei Du

摘要

Rice blast, a disease caused by Magnaporthe oryzae, significantly threatens global rice production. To improve the anti-M. oryzae activity of Streptomyces bikiniensis HD-087 metabolites, the effects of inducer, Magnaporthe oryzae acellular filtrate, on secondary metabolism of S. bikiniensis HD-087 were studied. The results showed that M. oryzae cell-free filtrate cultured for 96 h served as the most effective inducer, significantly enhancing the anti-M. oryzae activity of metabolites of S. bikiniensis HD-087 and increasing the diameter of the inhibitory zone by 2.96 mm. The inhibition rates of M. oryzae colony diameter and spore germination in the induced group were 12.39% and 39.6% higher than those in the non-induced group, respectively. Metabolomic profiling of strain HD-087 highlighted substantial differences between the induced and non-induced groups. At 48 h of fermentation, a total of 705 distinct metabolites were identified, while at 96 h this number decreased to 321. Moreover, induction markedly altered primary pathways such as the tricarboxylic acid cycle, amino acid biosynthesis, and fatty acid metabolism in S. bikiniensis HD-087. qPCR analysis showed that nrps genes and pks genes in the induced group were significantly up-regulated by 9.92 ± 0.51 and 2.71 ± 0.17 times, respectively, and biotin carboxylase activity was also increased 26.63%. These results provide a theoretical basis for using inducers to enhance the antimicrobial ability of Streptomyces.

Graphical Abstract