Scholarlink

Background <p>The association between the intestinal microbiota and colorectal cancer (CRC) has been extensively studied, with <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>, FN) being found in high abundance in colorectal cancer tissues. Previous research has emphasized the significant role of <i>F. nucleatum</i> in the occurrence of CRC. However, the impact of <i>F. nucleatum</i> on CRC liver metastasis has not been well understood.</p> Methods <p>The effects of <i>F. nucleatum</i> on metastasis ability of CRC cell were evaluated in vitro were examined by wound-healing assay and transwell assay. The mouse model of CRC liver metastasis was constructed by spleen injection, and the degree of liver metastasis was assessed by in vivo bioluminescence imaging. The gene expression changes in CRC cells after co-culture with <i>F. nucleatum</i> was analyzed through transcriptome sequencing. qRT-PCR and Western Blot assays were performed to validate the expression of related genes and proteins.</p> Results <p>The metastasis ability of CRC cells was significantly enhanced after co-culture with <i>F. nucleatum</i> in vitro. In the mouse model, <i>F. nucleatum</i> also promoted the development of liver metastasis in CRC. Mechanistically, <i>F. nucleatum</i> infection increased the expression of IL-8 by downregulated the level of miR-5692a, a regulatory microRNA of IL-8. This led to the activation of the ERK pathway and resulted in the epithelial-mesenchymal transition (EMT) of CRC cells.</p> Conclusions <p>Our results suggest that <i>F. nucleatum</i> promotes CRC liver metastasis by inducing epithelial-mesenchymal transition through the miR-5692a/IL-8 axis. These findings provide new insights for the prevention and treatment of colorectal cancer liver metastasis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fusobacterium nucleatum promotes colorectal cancer liver metastasis via miR-5692a/IL-8 axis by inducing epithelial-mesenchymal transition

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月6日

查看全文
  • Yulong Yu,
  • Han Yin,
  • Bili Wu,
  • Weiheng Zhao,
  • Yuan Wang,
  • Aifeina Aili,
  • Mu Yang,
  • Qianqian Yu,
  • Xianglin Yuan

摘要

Background

The association between the intestinal microbiota and colorectal cancer (CRC) has been extensively studied, with Fusobacterium nucleatum (F. nucleatum, FN) being found in high abundance in colorectal cancer tissues. Previous research has emphasized the significant role of F. nucleatum in the occurrence of CRC. However, the impact of F. nucleatum on CRC liver metastasis has not been well understood.

Methods

The effects of F. nucleatum on metastasis ability of CRC cell were evaluated in vitro were examined by wound-healing assay and transwell assay. The mouse model of CRC liver metastasis was constructed by spleen injection, and the degree of liver metastasis was assessed by in vivo bioluminescence imaging. The gene expression changes in CRC cells after co-culture with F. nucleatum was analyzed through transcriptome sequencing. qRT-PCR and Western Blot assays were performed to validate the expression of related genes and proteins.

Results

The metastasis ability of CRC cells was significantly enhanced after co-culture with F. nucleatum in vitro. In the mouse model, F. nucleatum also promoted the development of liver metastasis in CRC. Mechanistically, F. nucleatum infection increased the expression of IL-8 by downregulated the level of miR-5692a, a regulatory microRNA of IL-8. This led to the activation of the ERK pathway and resulted in the epithelial-mesenchymal transition (EMT) of CRC cells.

Conclusions

Our results suggest that F. nucleatum promotes CRC liver metastasis by inducing epithelial-mesenchymal transition through the miR-5692a/IL-8 axis. These findings provide new insights for the prevention and treatment of colorectal cancer liver metastasis.