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Background <p>Chicken egg yolk immunoglobulin Y (IgY) has shown potential in preventing pathogen infections. This study examined the effectiveness of dietary IgY supplementation at 6&#xa0;g/kg in preventing weaned piglets from developing diarrhoea after a challenge with <i>Escherichia coli</i> K88 (<i>E. coli</i> K88). The experiment employed a 2 × 2 factorial design. Twenty-four healthy 17-day-old piglets with similar body weights (4.63 ± 0.39&#xa0;kg) were selected and divided into 4 groups, each with 6 replicates (<i>n</i> = 6). The experimental period lasted 11 days. The control group (CON) and the <i>E. coli</i> K88 group (ECON) were fed a basal diet supplemented with 6&#xa0;g/kg of common yolk powder. Meanwhile, the anti-<i>E. coli</i> K88 IgY group (IgY) and the <i>E. coli</i> K88 IgY group (EIgY) received a diet supplemented with 6&#xa0;g/kg of anti-<i>E. coli</i> K88 IgY. From days 8 to 11 of the formal trial, piglets in the CON and IgY groups received an equal volume of normal saline (NS), while the ECON and EIgY groups were orally administered 10 mL of NS containing approximately 1 × 10<sup>10</sup> CFU/mL of <i>E. coli</i> K88.</p> Results <p>Results showed that dietary 6&#xa0;g/kg anti-<i>E. coli</i> K88 IgY supplementation increased the IL-10 protein expression level in the jejunum tissue, villus height (VH), the villus height to crypt depth ratio (V: C), sucrase activities, and reduced colonic <i>E. coli</i> counts, diarrhea incidence, and D-LA levels in <i>E. coli</i> K88-infected piglets (<i>P</i> &lt; 0.05). Meanwhile, the IgY diet increased Claudin-1 protein levels and decreased IL-8 levels in the jejunum tissue of <i>E. coli</i> K88-challenged piglets (<i>P</i> &lt; 0.05).</p> Conclusions <p>The dietary 6&#xa0;g/kg anti-<i>E. coli</i> K88 IgY supplementation can reduce intestinal structural damage and inflammation, improving growth by enhancing passive immunity and intestinal barrier function in <i>E coli</i> K88-challenged piglets.</p> Graphical Abstract <p></p>

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Dietary anti- Escherichia coli K88 IgY enhances immune response and intestinal barrier function in piglets challenged with Escherichia coli K88

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月4日

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  • Yueyao Xing,
  • Miaomiao Bai,
  • Junhong Liu,
  • Ruixian Hu,
  • Pingli He,
  • Bie Tan,
  • Hongnan Liu

摘要

Background

Chicken egg yolk immunoglobulin Y (IgY) has shown potential in preventing pathogen infections. This study examined the effectiveness of dietary IgY supplementation at 6 g/kg in preventing weaned piglets from developing diarrhoea after a challenge with Escherichia coli K88 (E. coli K88). The experiment employed a 2 × 2 factorial design. Twenty-four healthy 17-day-old piglets with similar body weights (4.63 ± 0.39 kg) were selected and divided into 4 groups, each with 6 replicates (n = 6). The experimental period lasted 11 days. The control group (CON) and the E. coli K88 group (ECON) were fed a basal diet supplemented with 6 g/kg of common yolk powder. Meanwhile, the anti-E. coli K88 IgY group (IgY) and the E. coli K88 IgY group (EIgY) received a diet supplemented with 6 g/kg of anti-E. coli K88 IgY. From days 8 to 11 of the formal trial, piglets in the CON and IgY groups received an equal volume of normal saline (NS), while the ECON and EIgY groups were orally administered 10 mL of NS containing approximately 1 × 1010 CFU/mL of E. coli K88.

Results

Results showed that dietary 6 g/kg anti-E. coli K88 IgY supplementation increased the IL-10 protein expression level in the jejunum tissue, villus height (VH), the villus height to crypt depth ratio (V: C), sucrase activities, and reduced colonic E. coli counts, diarrhea incidence, and D-LA levels in E. coli K88-infected piglets (P < 0.05). Meanwhile, the IgY diet increased Claudin-1 protein levels and decreased IL-8 levels in the jejunum tissue of E. coli K88-challenged piglets (P < 0.05).

Conclusions

The dietary 6 g/kg anti-E. coli K88 IgY supplementation can reduce intestinal structural damage and inflammation, improving growth by enhancing passive immunity and intestinal barrier function in E coli K88-challenged piglets.

Graphical Abstract