Scholarlink

Background <p>Canine diarrhea with long course and a certain mortality rate depends mainly on supportive treatment. Oral recombinant epidermal growth factor (EGF) can stimulate intestinal development and reduce diarrhea incidence in human and other animals, but few related studies have been done in canine.</p> Results <p>Here, we firstly constructed a recombinant <i>lactococcus lactis</i> (<i>L. lactis</i>) expressing bioactive canine EGF (cEGF), and then explored its effect on cell proliferation, intestinal injury repair and inflammatory factor secretion in diarrheal mice and canines. The results showed that cEGF promoted the proliferation of CMT-1211 cells, that cEGF-expressing <i>L. lactis</i> strain recovered the damaged mucous structure and suppressed the inflammatory response, potentially by activating EGF receptor and its downstream AKT and ERK1/2 signals, thereby alleviating diarrhea in mice and canine.</p> Conclusions <p>We first generated a cEGF-expressing <i>L. lactis</i> strain and demonstrated its potential to repair intestinal injury and alleviate diarrhea in mice and canines, and thus cEGF-expressing <i>L. lactis</i> could be a new therapy strategy for animal diarrhea.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Canine epidermal growth factor-expressing Lactococcus lactis promotes intestinal injury repair and alleviates diarrhea in canine

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月6日

查看全文
  • Yinghui Gong,
  • Huiqi Cui,
  • Jinxin Zhang,
  • Wen Feng,
  • Yongjie Yuan,
  • Ganzhen Deng,
  • Yucheng He

摘要

Background

Canine diarrhea with long course and a certain mortality rate depends mainly on supportive treatment. Oral recombinant epidermal growth factor (EGF) can stimulate intestinal development and reduce diarrhea incidence in human and other animals, but few related studies have been done in canine.

Results

Here, we firstly constructed a recombinant lactococcus lactis (L. lactis) expressing bioactive canine EGF (cEGF), and then explored its effect on cell proliferation, intestinal injury repair and inflammatory factor secretion in diarrheal mice and canines. The results showed that cEGF promoted the proliferation of CMT-1211 cells, that cEGF-expressing L. lactis strain recovered the damaged mucous structure and suppressed the inflammatory response, potentially by activating EGF receptor and its downstream AKT and ERK1/2 signals, thereby alleviating diarrhea in mice and canine.

Conclusions

We first generated a cEGF-expressing L. lactis strain and demonstrated its potential to repair intestinal injury and alleviate diarrhea in mice and canines, and thus cEGF-expressing L. lactis could be a new therapy strategy for animal diarrhea.