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<p>Antimicrobial peptide BmKn2-7, a rationally optimized derivative of the scorpion venom peptide BmKn2, shows strong potential as an antibiotic alternative for controlling <i>Vibrio parahaemolyticus</i> in aquaculture. This study evaluated its antibacterial mechanisms and in vivo efficacy in <i>Litopenaeus vannamei</i> through biochemical assays, transcriptomic profiling, and challenge experiments. BmKn2-7 exhibited potent in-vitro activity (MIC = 125&#xa0;µg/mL) and disrupted bacterial homeostasis by increasing membrane permeability, inducing oxidative stress, and inhibiting P-type ATPase. RNA-seq analysis revealed extensive transcriptional reprogramming, including the downregulation of quorum-sensing, virulence, and β-lactam resistance–associated genes. In vivo, BmKn2-7 significantly improved shrimp survival following <i>V. parahaemolyticus</i> infection and selectively reduced <i>Vibrio</i> abundance in the gut without compromising overall microbiota diversity. Together, these findings demonstrate that BmKn2-7 acts through complementary bactericidal and anti-virulence pathways while promoting intestinal microbial resilience, supporting its potential as a promising antimicrobial candidate for reducing vibriosis risk in shrimp aquaculture.</p>

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Effect of the antimicrobial peptide BmKn2-7 in inhibiting Vibrio parahaemolyticus and its role in improving microbiota, immunity and Vibrio resistance in Litopenaeus vannamei

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年1月16日

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  • Qihang Liang,
  • Qi Wang,
  • Pinqi Chi,
  • Depeng Fan,
  • Beiping Tan,
  • Shiwei Xie,
  • Junming Deng,
  • Hongyu Liu,
  • Ling Zeng

摘要

Antimicrobial peptide BmKn2-7, a rationally optimized derivative of the scorpion venom peptide BmKn2, shows strong potential as an antibiotic alternative for controlling Vibrio parahaemolyticus in aquaculture. This study evaluated its antibacterial mechanisms and in vivo efficacy in Litopenaeus vannamei through biochemical assays, transcriptomic profiling, and challenge experiments. BmKn2-7 exhibited potent in-vitro activity (MIC = 125 µg/mL) and disrupted bacterial homeostasis by increasing membrane permeability, inducing oxidative stress, and inhibiting P-type ATPase. RNA-seq analysis revealed extensive transcriptional reprogramming, including the downregulation of quorum-sensing, virulence, and β-lactam resistance–associated genes. In vivo, BmKn2-7 significantly improved shrimp survival following V. parahaemolyticus infection and selectively reduced Vibrio abundance in the gut without compromising overall microbiota diversity. Together, these findings demonstrate that BmKn2-7 acts through complementary bactericidal and anti-virulence pathways while promoting intestinal microbial resilience, supporting its potential as a promising antimicrobial candidate for reducing vibriosis risk in shrimp aquaculture.