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Background <p>Juvenile hormone (JH) is synthesized by the <i>corpora allata</i> (CA) and controls development and reproduction in insects. We recently used CRISPR/Cas9 to establish a line lacking the enzyme that catalyzes the final step of JH biosynthesis in mosquitoes, a P450 epoxidase. The CA of the <i>epox</i><sup><i>−/−</i></sup> mutants do not synthesize epoxidized JH III but methyl farneosate (MF), a weak agonist of the JH receptor. Female <i>epox</i><sup><i>−/−</i></sup> mosquitoes have reduced JH signaling and show a substantial loss of reproductive fitness. To understand the molecular basis of this loss of fitness, we constructed ovarian mRNA libraries of <i>Ae. aegypti</i> of the Orlando strain wild-type (<i>WT</i>) and <i>epoxidase</i> null mutants (<i>epox</i><sup><i>−</i>/<i>−</i></sup>) and investigated differential expression of reproductive genes.</p> Results <p>We performed triplicate RNA-seq analyses of female <i>WT</i> and <i>epox</i><sup><i>−/−</i></sup> ovaries dissected at four critical stages of oogenesis: Ovaries from newly eclosed females (0h), sugar-fed females at 4&#xa0;days post-eclosion (4d SF), females 16h (16h BF), and 48 h after a blood meal (48h BF). Silencing of epoxidase resulted in a drastic change in the expression of thousands of genes.</p> Conclusions <p>Our results suggest that epoxidase deficiency leads to a reduction in JH signaling that has significant effects on <i>Ae. aegypti</i> ovarian transcriptome profiles. Ecdysteroid titers are dysregulated in the mutants, leading to a significant delay in the expression of vitelline membrane genes and other transcripts. We discovered changes in the expression of 230 long non-coding RNAs&#xa0;(lncRNAs) that may play an important role in the regulation of ovarian genes.</p>

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Ovarian transcriptome analyses indicate that weak juvenile hormone signaling underlies the molecular basis of oogenesis deficiencies in mosquitoes

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  • 发表日期：

    2025年6月9日

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  • Monika M. Wiśniewska,
  • Martin Kolísko,
  • Petra Berková,
  • Martin Moos,
  • Houda O. Maaroufi,
  • Fernando G. Noriega,
  • Marcela Nouzova

摘要

Background

Juvenile hormone (JH) is synthesized by the corpora allata (CA) and controls development and reproduction in insects. We recently used CRISPR/Cas9 to establish a line lacking the enzyme that catalyzes the final step of JH biosynthesis in mosquitoes, a P450 epoxidase. The CA of the epox−/− mutants do not synthesize epoxidized JH III but methyl farneosate (MF), a weak agonist of the JH receptor. Female epox−/− mosquitoes have reduced JH signaling and show a substantial loss of reproductive fitness. To understand the molecular basis of this loss of fitness, we constructed ovarian mRNA libraries of Ae. aegypti of the Orlando strain wild-type (WT) and epoxidase null mutants (epox−/−) and investigated differential expression of reproductive genes.

Results

We performed triplicate RNA-seq analyses of female WT and epox−/− ovaries dissected at four critical stages of oogenesis: Ovaries from newly eclosed females (0h), sugar-fed females at 4 days post-eclosion (4d SF), females 16h (16h BF), and 48 h after a blood meal (48h BF). Silencing of epoxidase resulted in a drastic change in the expression of thousands of genes.

Conclusions

Our results suggest that epoxidase deficiency leads to a reduction in JH signaling that has significant effects on Ae. aegypti ovarian transcriptome profiles. Ecdysteroid titers are dysregulated in the mutants, leading to a significant delay in the expression of vitelline membrane genes and other transcripts. We discovered changes in the expression of 230 long non-coding RNAs (lncRNAs) that may play an important role in the regulation of ovarian genes.