Background <p>It is known that quality studies increase satisfaction, positively affect productivity and support corporate development. This methodological study aims to develop a scale that measures the attitudes of healthcare professionals toward quality studies.</p> Methods <p>A methodological study using instrument-development and instrument verification phases. The research universe was composed of health workers working in 5 hospitals in Istanbul (<i>N</i> = 6308), and the sample was composed of health workers who agreed to participate in the research (<i>n</i> = 1013). The researchers followed the scale development stages: item pooling, expert opinion, preliminary application, validity, and reliability.</p> Results <p>KMO and Bartlett’s test values of scale showed that the dataset was convenient for factor analyses (KMO = 0.976, Chi-Square = 20624.814, df = 861). In exploratory factor analysis, the 42 items comprising scale were distributed in three subscales. The confirmatory factor analysis revealed that the scale was in sufficient model fit. Cronbach’s alpha of the total scale was 0.976.</p> Conclusion <p>The “Attitude of Health Workers towards Quality Studies” scale was determined to be valid and reliable. This scale could serve as a comprehensive tool for evaluating quality initiatives in healthcare. It could possess the capacity to bring together different institutions (public, private, university) in a way that fosters mutual growth. The assessment of healthcare quality initiatives could pave the way for inclusive improvements to be planned. Through all the planned enhancements, both patient and employee satisfaction can be enhanced.</p>