Background <p>Enteral nutrition interruptions are common in clinical settings, with intensive care unit (ICU) nurses being key in overseeing these procedures. Yet, there is limited evidence on the knowledge, attitudes, and practices (KAP) of ICU nurses in Shandong Province regarding enteral nutrition interruptions.</p> Objective <p>This study seeks to assess the knowledge, attitudes, and practices related to the cessation of enteral nutrition among ICU nurses in Shandong Province and identify influencing factors.</p> Methods <p>A cross-sectional survey was carried out among ICU nurses in 18 tertiary hospitals in Shandong Province between February and March 2025. Through stratified random sampling, 370 eligible nurses were enlisted. The participants completed a two-part self-administered questionnaire. The first part gathered demographic data such as sex, age, job title, years of experience(just years as an ICU nurse), ICU type, specialist certification status, nutritional support team membership, and training pertaining to enteral nutrition interruption (EN). The second part comprised the validated ICU Care Enteral Nutrition Interruption Knowledge, Attitude, and Practice (KAP) Scale. Data analysis was performed using SPSS 20, reporting the median and interquartile range for non-normally distributed data descriptively. Univariate analysis and multiple linear regression were employed to identify factors influencing the KAP score. Statistical significance was set at <i>P</i> &lt; 0.05.</p> Results <p>The total Knowledge, Attitudes, and Practices (KAP) score of intensive care unit (ICU) nurses in Shandong Province was 175 (161–187), with sub-scores of 56 (49–61) for knowledge, 49 (40–50) for attitudes, and 72 (68–80) for practices.Multiple linear regression analysis indicated that several factors influenced ICU nurses’ knowledge of enteral nutrition interruption, including nursing category, ICU specialist nurse qualification, and specific training on receiving enteral nutrition interruption (<i>p</i> &lt; 0.05). Age and nurse title were found to impact nurses’ attitudes toward enteral nutrition interruption (<i>p</i> &lt; 0.05). Moreover, knowledge, attitude towards enteral nutrition interruption, nurse title, and special training on enteral nutrition interruption were identified as factors influencing nurses’ behavior related to enteral nutrition interruption (<i>p</i> &lt; 0.05).</p> Conclusion <p>The study indicated a generally high level of knowledge, attitudes, and practices concerning enteral nutrition interruption among ICU nurses in Shandong Province, although knowledge scores suggested potential for enhancement. Various factors influenced the knowledge, attitude, and behavior of ICU nurses in Shandong Province regarding enteral nutrition interruption. Specifically, knowledge was influenced by nursing category, specialty qualification, and training. Attitude was associated with age and nurse title. Moreover, practical behavior was influenced by knowledge, attitude, nurse title, and training.</p> Clinical trial number <p>Not applicable.</p>