Background <p>Orientation programs support the transition of novice nurses into their professional roles and facilitate their integration into the team.</p> Aim <p>This research was conducted to examine the effect of a structured orientation program on teamwork attitudes and nurse outcomes among newly graduated nurses.</p> Methods <p>The study employed a quasi-experimental pre-test–post-test design. The study was conducted with novice nurses at a university hospital (intervention group: <i>n</i> = 112, control group: <i>n</i> = 188). The control group received the standard orientation program of the institution, while the intervention group participated in a structured orientation program. The study examined teamwork attitude, nurses’ job satisfaction, and their intention to leave the profession. An introductory information and work characteristics form and the Teamwork Attitudes Questionnaire were used as data collection tools, while job satisfaction and intention to leave the profession were evaluated with a single-item measure. For data analysis, descriptive statistics and the independent samples t-test were applied. In addition, the Transparent Reporting of Evaluations with Non-randomized Designs reporting guideline checklist was used.</p> Results <p>The study found that while there were no differences in variables between the control and intervention groups at baseline, by the sixth month, job satisfaction among nurses in the control group had decreased, while those in the intervention group had higher job satisfaction. However, no significant changes were found in teamwork attitudes or intention to leave the profession.</p> Conclusions <p>These results suggest that multi-intervention orientation programs designed to address the competencies can be effective in increasing job satisfaction for novice nurses transitioning to their professional roles. Further research is recommended on novice nurses’ teamwork attitudes and other variables affecting their intention to leave the profession.</p>