Background <p>This study explores Danish patients’ perceptions of male nurses and how gender influences experiences of vulnerability in nursing care. Using a mixed-methods design, data were collected from 101 participants through an anonymous online questionnaire. The results showed that most patients had no gender preference in routine nursing care. However, gender sometimes became important in intimate care situations involving bodily exposure. Participants described vulnerability as influenced by physical, emotional, and cultural factors. Despite this, most respondents reported positive experiences with male nurses, and professional communication, consent, and respect for boundaries were important for maintaining patient dignity and trust.</p> Introduction <p>Patient dignity and autonomy are central principles in nursing, particularly in situations involving bodily exposure and intimate care. Previous research suggests that patients may express gender preferences for nurses mainly in intimate care contexts, while gender is generally irrelevant in routine care. This study aimed to explore Danish patients’ perceptions of male nurses and examine how gender becomes meaningful in situations involving vulnerability.</p> Methods <p>The study used a convergent mixed methods design. Data were collected through an anonymous online questionnaire distributed via social media. A total of 101 participants with healthcare experience completed the survey. Quantitative data were analyzed using descriptive statistics, and qualitative responses were analyzed using thematic analysis, with integration of the two datasets during interpretation.</p> Results <p>Most participants reported <b>no</b> general gender preference in routine nursing care. However, some participants stated that gender became relevant in intimate care situations involving bodily exposure. Three themes emerged: (1) Gender as a situational trigger of vulnerability. (2) Bodily, emotional, and cultural dimensions of vulnerability. (3) Professional communication and boundary awareness supporting dignity and trust. (4) Overall, most participants reported feeling safe and having positive experiences with male nurses.</p> Discussion <p>Gender was not a general determinant of patient preference but became meaningful mainly in situations involving intimate bodily exposure. Experiences of vulnerability were shaped by bodily awareness, emotional reactions, and cultural norms. However, communication, consent-seeking, and professional professionalism helped maintain patient trust, safety, and dignity in care encounters.</p> Clinical trial number <p>Not applicable.</p>