Background <p>Psychological well-being, helpfulness, and tolerance are fundamental concepts for nursing students’ professional identity development, human-centered care approach, and quality healthcare.</p> Objective <p>This study was conducted to examine the relationship between psychological well-being, helpfulness and tolerance tendency in nursing students.</p> Methods <p>The descriptive and correlational study was conducted between April and July 2022 with the voluntary participation of 753 students studying in the nursing departments of three public and one private universities in Türkiye. The data were collected with the Information Form, Psychological Well-Being Scale, Helpfulness Scale, Tolerance Tendency Scale Form for Adults and tested with t-test, Kruskal Wallis H, ANOVA, Pearson Correlation, Multiple Linear Regression Analyses.</p> Results <p>The psychological well-being scale score of the students was 5.31 ± 0.94, the helpfulness scale score was 4.26 ± 0.51 and the tolerance tendency scale score was 3.62 ± 0.65. While students’ tolerance tendency (β = 0.389), psychological well-being (β = 0.240), being female (β = 0.114), liking the nursing department (β = 0.077) and having good relationships with their friends (β = 0.113) significantly positively affected their helpfulness scale scores, having a nuclear family structure negatively affected them (β=-0.068) (<i>p</i> &lt; 0.05).</p> Conclusion <p>Students had high levels of psychological well-being and helpfulness and moderate levels of tolerance tendency. There is a significant relationship between these three concepts, and tolerance tendency has had the greatest impact on helpfulness. For the sustainability of students’ helpfulness, simulation and group trainings and games can be planned to improve their tolerance tendencies.</p>