Background <p>This study aimed to examine the knowledge and behaviours of the intensive care nurses about pain.</p> Methods <p>This descriptive and cross-sectional study was conducted with 72 intensive care nurses. ‘Nurse Information Form’, ‘Nurses Knowledge and Behavioral Questionnaire’ were used for data collection. The data collection process was conducted face-to-face by the researchers. Descriptive statistics and the Mann-Whitney U Test analysis were used in the analysis of the data.</p> Results <p>It was determined that 62.5% of the intensive care nurses participating in our study were 30 years old or younger. 100% of the nurses participating in the study used a scale in assessing pain, but only 21.6% of the nurses used the behavioral pain scale. It was determined that the level of knowledge of the nurses about pain was moderate. It was determined that there was a significant difference between the gender, nurses pain-related training status, education level and Nurses Pain-Related Knowledge and Behavior Questionnaire scores.</p> Conclusion <p>It was determined that the knowledge level of the nurses working in the intensive care unit was moderate and very few of the nurses used the behavioral pain scale for non-communicative patients. This finding highlights the need to increase evidence-based training for nurses and to promote the use of behavioral pain scales, particularly in non-communicative patients.</p> Clinical trial number <p>Not applicable.</p>