Aim <p>This study investigates the association between green human resource practices and organizational environmental performance among nurses at Alexandria Main University Hospital.</p> Design <p>A cross-sectional descriptive design following STROBE guidelines examined the relationship between green human resource practices and organizational environmental performance among nurses.</p> Methods and tools <p>Staff nurses employed in all inpatient medical, surgical, and critical care units at Alexandria Main University Hospital (<i>n</i> = 745) comprised the participants, representing all target demographics. The nurses filled out the Organizational Environmental Performance Scale and the Green Human Resources Practices Questionnaire. Age, gender, education, and nursing experience were among the demographic details gathered. The data collection period was three months, from February 1st, 2023, to May 1st, 2023. Every query from the nurses was addressed, and justifications were provided. The data was analyzed using statistical techniques such as stepwise regression, ANOVA, t-tests, and Pearson correlation.</p> Results <p>60.7% of the studied nurses perceived low Green Human Resources Practices (63.27 ± 8.14). Furthermore, the majority of the nurses who participated in the study (90.1%) believe that environmental performance is at a moderate level, with a mean score of 73.86 ± 15.54. According to linear regression, the Green Human Resources Practices of the nurses under study can account for about 17.5% of the explained variance of their perceived environmental performance; this is a significant model (F = 157.939 &amp; <i>p</i> &lt; 0.001).</p> Conclusion <p>The study highlights the importance of the perceived adoption of Green Human Resources Practices in supporting environmental performance. Addressing demographic factors and fostering a supportive work environment is crucial for optimizing organizational environmental performance.</p> Nursing implications <p>Insights from this study can enlighten battered interventions and policy pronouncements to augment nursing practice, organizational growth, and healthcare outcomes in Egypt. Green HR practices have consequences for nursing, such as enabling nurses to spearhead sustainability projects, encouraging environmentally friendly patient care techniques, and raising employee involvement through incentives and training. Nurses can make a substantial contribution to better environmental performance in healthcare settings by incorporating these practices. In the end, this promotes a sustainable culture that is advantageous to patients and the environment.</p> Clinical trial number <p>Not applicable.</p>