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Background <p>Microscope operation is a core skill in morphological laboratory education. However, traditional instructional approaches often face limitations in equipment accessibility, procedural guidance, and hands-on opportunities. With the advancement of virtual simulation technologies, new pathways have emerged for improving practical skill training. This study presents the development of VirtuScope, a high-fidelity virtual microscope simulation system, and explores its application value in histology education.</p> Methods <p>VirtuScope was developed as a standalone virtual simulation system for microscopes, featuring 1:1 3D structural reconstruction and high-fidelity simulation of physical interactions and optics, powered by advanced human-computer interaction and computer graphics. Key simulation features include focus adjustment, objective switching, and light source control. In the instructional design, VirtuScope was integrated into a blended teaching model combining video guidance, self-directed practice, and progressive transition to real microscope use. Student feedback and learning outcomes were evaluated using pre- and post-course questionnaires and knowledge tests.</p> Results <p>Students rated the system highly in terms of image clarity, interactivity, and instructional support. Most participants reported improved understanding of microscope structure and operation, and over 90% expressed willingness to use VirtuScope in future learning. Knowledge test scores showed significant post-course improvement (<i>p</i> &lt; 0.001), and the overall recommendation rate reached 98.52%.</p> Conclusion <p>VirtuScope demonstrates strong adaptability and educational support capacity. Its visual, interactive, and repeatable features enhance learning efficiency and practical competence, offering a scalable complement to traditional microscopy training. Moreover, it provides a feasible solution for institutions with limited laboratory resources, extending access to high-quality microscopy education across diverse learning environments.</p>

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VirtuScope: development of a high-fidelity virtual microscope simulation system for histology education

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月21日

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  • Weixin Huang,
  • Xiaoting Li,
  • Xiaotong Cao,
  • Kaiqi Zhang,
  • Yuwei Lin,
  • Minwen Zhan,
  • Jingyan Chen,
  • Zhongjing Su,
  • Yang Gao

摘要

Background

Microscope operation is a core skill in morphological laboratory education. However, traditional instructional approaches often face limitations in equipment accessibility, procedural guidance, and hands-on opportunities. With the advancement of virtual simulation technologies, new pathways have emerged for improving practical skill training. This study presents the development of VirtuScope, a high-fidelity virtual microscope simulation system, and explores its application value in histology education.

Methods

VirtuScope was developed as a standalone virtual simulation system for microscopes, featuring 1:1 3D structural reconstruction and high-fidelity simulation of physical interactions and optics, powered by advanced human-computer interaction and computer graphics. Key simulation features include focus adjustment, objective switching, and light source control. In the instructional design, VirtuScope was integrated into a blended teaching model combining video guidance, self-directed practice, and progressive transition to real microscope use. Student feedback and learning outcomes were evaluated using pre- and post-course questionnaires and knowledge tests.

Results

Students rated the system highly in terms of image clarity, interactivity, and instructional support. Most participants reported improved understanding of microscope structure and operation, and over 90% expressed willingness to use VirtuScope in future learning. Knowledge test scores showed significant post-course improvement (p < 0.001), and the overall recommendation rate reached 98.52%.

Conclusion

VirtuScope demonstrates strong adaptability and educational support capacity. Its visual, interactive, and repeatable features enhance learning efficiency and practical competence, offering a scalable complement to traditional microscopy training. Moreover, it provides a feasible solution for institutions with limited laboratory resources, extending access to high-quality microscopy education across diverse learning environments.