Inroduction <p>The Simulation-based Interprofessional Teamwork Assessment Tool (SITAT) is a valuable instrument for evaluating individual performance within interprofessional teams.</p> Aim <p>This study aimed to translate and validate the SITAT into Turkish (SITAT-TR) to enhance interprofessional education and teamwork assessments in the Turkish context.</p> Methods <p>This study was designed as an adaptation study in a descriptive research design. Ethical approval was obtained for the study. The process of Turkish translation and cross-cultural adaptation was completed. Subsequently, a simulation scenario was developed. The scenario was performed and recorded by standardized patients representing professionals in different roles. These videos were then reviewed by students from various professions to conduct validity and reliability studies.</p> Results <p>This study evaluated 345 students from five professions at Süleyman Demirel University, using the SITAT-TR scale. Psychometric analysis showed strong validity with high content validity indices (I-CVI: 0.95–1.00; S-CVI/Ave, 0.98) and internal consistency (Cronbach’s alpha = 0.915). Suitability of factor analysis was confirmed by a high KMO value (0.940) and significant Bartlett’s test results, supporting a unidimensional structure. Simulation-based competency assessments revealed mostly ‘proficient’ ratings, with significant differences between physicians and dietitians in certain tests (SP2 and SP4). These findings highlight the reliability of the SITAT-TR and its generally high competency levels within interprofessional teamwork in healthcare settings.</p> Conclusion <p>This study showed that the Turkish version of the Simulation-Based Interprofessional Teamwork Assessment Tool (SITAT) can be used as a valid and reliable measurement tool.</p>