Scholarlink

Background <p><i>Capparis decidua</i> (Forssk.) Edgew., a perennial shrub prevalent in arid Africa and Asia, holds prominent status in Sudanese traditional medicine for inflammation, infections, and scorpion stings. Despite extensive ethnomedicinal use, particularly of root bark, comprehensive scientific validation remains limited. This study evaluated <i>C. decidua</i> root bark extracts for anti-inflammatory and antimicrobial activities through integrated in vitro, GC-MS, and in silico approaches.</p> Methods <p>Root bark underwent 85% methanolic (MeOH) extraction followed by fractionation with petroleum ether (Pet.ether), ethyl acetate (EtOAc), and <i>n</i>-butanol (<i>n</i>- BuOH). The Extracts were assessed as anti-inflammatory and antimicrobial via protein denaturation assay and via well-diffusion assays, respectively. The phytoconstituents were investigated via GC-MS analysis (non-derivatized, NIST ≥ 85% match), and the molecular docking was done against pathogen protein targets.</p> Results <p>The GC-MS analysis revealed to identification of 23 metabolites (98.58% total peak area, peaks 3–25; peaks 1–2 excluded as siloxane artifacts), with 9-octadecenoic acid (Z)-2-hydroxy-1-(hydroxymethyl)ethyl ester (18.13%), 1-H-purin-6-amine[(2-fluorophenyl)methyl] (13.73%), oleic acid 3-(octadecyloxy)propyl ester (13.21%), and 9-octadecenoic acid (Z) (11.03%) predominant. The 85% methanolic extract displayed superior antimicrobial activity against all tested pathogens (<i>Staphylococcus aureus</i> 29 ± 2&#xa0;mm; <i>B. subtilis</i> 25 ± 2&#xa0;mm; <i>K. pneumoniae</i> 24 ± 1&#xa0;mm; <i>S. typhi</i> 22 ± 1&#xa0;mm; <i>C. albicans</i> 24 ± 2&#xa0;mm; <i>A. niger</i> 19 ± 1&#xa0;mm; <i>n</i> = 3), outperforming gentamicin and fluconazole controls against key strains. <i>n</i>BuOH/EtOAc fractions showed strong activity (21–29&#xa0;mm). Anti-inflammatory IC₅₀: MeOH 163.72&#xa0;µg/mL, <i>n</i>-BuOH 178.22&#xa0;µg/mL, EtOAc 234.59&#xa0;µg/mL vs. diclofenac 121.55&#xa0;µg/mL. Docking: 1-H-purin-6-amine [(2-fluorophenyl) methyl] (-9.5 to -10.0&#xa0;kcal/mol).</p> Conclusion <p><i>Capparis decidua</i> root bark demonstrates significant anti-inflammatory and broad-spectrum antimicrobial potential, validating Sudanese traditional use. In vivo studies, compound isolation, mechanistic assays, and toxicity profiling are warranted.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Phytochemical profiling, bioactivity and in silico studies of Capparis decidua Edgew (Forssk.) root bark extracts

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年8月4日

查看全文
  • Mazen M. M. Taha,
  • Nazik M. E. Mustafa,
  • Abdalkarim M. M. Salih,
  • Saad M. H. Ayoub,
  • EL-Sayed S. Abdel-Hameed,
  • Abdullah E. Gouda,
  • Ezzat E. A. Osman,
  • Mohamed A. Shemis

摘要

Background

Capparis decidua (Forssk.) Edgew., a perennial shrub prevalent in arid Africa and Asia, holds prominent status in Sudanese traditional medicine for inflammation, infections, and scorpion stings. Despite extensive ethnomedicinal use, particularly of root bark, comprehensive scientific validation remains limited. This study evaluated C. decidua root bark extracts for anti-inflammatory and antimicrobial activities through integrated in vitro, GC-MS, and in silico approaches.

Methods

Root bark underwent 85% methanolic (MeOH) extraction followed by fractionation with petroleum ether (Pet.ether), ethyl acetate (EtOAc), and n-butanol (n- BuOH). The Extracts were assessed as anti-inflammatory and antimicrobial via protein denaturation assay and via well-diffusion assays, respectively. The phytoconstituents were investigated via GC-MS analysis (non-derivatized, NIST ≥ 85% match), and the molecular docking was done against pathogen protein targets.

Results

The GC-MS analysis revealed to identification of 23 metabolites (98.58% total peak area, peaks 3–25; peaks 1–2 excluded as siloxane artifacts), with 9-octadecenoic acid (Z)-2-hydroxy-1-(hydroxymethyl)ethyl ester (18.13%), 1-H-purin-6-amine[(2-fluorophenyl)methyl] (13.73%), oleic acid 3-(octadecyloxy)propyl ester (13.21%), and 9-octadecenoic acid (Z) (11.03%) predominant. The 85% methanolic extract displayed superior antimicrobial activity against all tested pathogens (Staphylococcus aureus 29 ± 2 mm; B. subtilis 25 ± 2 mm; K. pneumoniae 24 ± 1 mm; S. typhi 22 ± 1 mm; C. albicans 24 ± 2 mm; A. niger 19 ± 1 mm; n = 3), outperforming gentamicin and fluconazole controls against key strains. nBuOH/EtOAc fractions showed strong activity (21–29 mm). Anti-inflammatory IC₅₀: MeOH 163.72 µg/mL, n-BuOH 178.22 µg/mL, EtOAc 234.59 µg/mL vs. diclofenac 121.55 µg/mL. Docking: 1-H-purin-6-amine [(2-fluorophenyl) methyl] (-9.5 to -10.0 kcal/mol).

Conclusion

Capparis decidua root bark demonstrates significant anti-inflammatory and broad-spectrum antimicrobial potential, validating Sudanese traditional use. In vivo studies, compound isolation, mechanistic assays, and toxicity profiling are warranted.