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<p>Quercetin (QCT) is a dietary flavonoid with reported antioxidant and neuroprotective properties, but direct comparisons with established antioxidants, such as ascorbic acid (AA), under chemically induced neurotoxic stress are limited. We compared Quercetin and AA in adult <i>D. melanogaster</i> using a manganese (Mn)-sensitized model to capture Parkinson-like motor and biochemical vulnerability. Treatment groups comprised: Control; Mn (15 mM); AA (10 mM); Quercetin 0.5 mM/3.0 mM; AA + Mn; Quercetin 0.5 mM/3.0 mM + Mn. Each vial represented an independent biological replicate (mixed sex); biochemical and genomic assays were performed using <i>n</i> = 4 vials/group. Outcomes included survival, locomotor performance (RING), redox endpoints (GSH/GSSG, H₂O₂, MDA, SOD, CAT), neuromodulators (AChE, dopamine), DNA fragmentation index (DFI), and advanced glycation end-products (AGEs). Omnibus tests (ANOVA or Kruskal-Wallis) were followed by Tukey HSD or Dunn-style pairwise comparisons; two pre-specified pooled contrasts were tested with Welch comparisons and effect sizes reported (mean differences, 95% CI, Cohen’s d); pooled p-values were adjusted (Holm, BH). Quercetin and AA produced comparable protective effects, as both preserved climbing behaviour and partially restored the redox balance versus Mn. One Tukey-adjusted pairwise result reached significance (Control vs. quercetin low + Mn for DFI, p_adj = 0.049). A pooled contrast showed reduced H₂O₂ for Quercetin (Q_all vs. AA_all: mean diff = 0.498 µmol·g⁻¹, 95% CI 0.153–0.844; Welch t(13.27) = 3.11, <i>p</i> = 0.0082; d ≈ 1.38), but this and other pooled signals did not survive conservative family-wise correction across all pooled tests. In conclusion, although they are overall comparable, our data suggest that quercetin may have a superior, specific effect on reducing H₂O₂ and protecting DNA integrity (DFI). These endpoints are now prioritized for confirmatory studies.</p> Graphical Abstract <p></p>

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Quercetin parallels ascorbic acid in neuroprotection against manganese-induced toxicity in Drosophila melanogaster

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月15日

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  • Tolulope T. Arogundade,
  • Oluwasegun D. Olatomide,
  • David A. Adeleye,
  • Eberechi Nwoke,
  • Vanessa C. Adim,
  • Ayodele Omotefe,
  • Adedamola A. Bayo-Olugbami,
  • Dayo R. Omotoso,
  • Ismail Gbadamosi

摘要

Quercetin (QCT) is a dietary flavonoid with reported antioxidant and neuroprotective properties, but direct comparisons with established antioxidants, such as ascorbic acid (AA), under chemically induced neurotoxic stress are limited. We compared Quercetin and AA in adult D. melanogaster using a manganese (Mn)-sensitized model to capture Parkinson-like motor and biochemical vulnerability. Treatment groups comprised: Control; Mn (15 mM); AA (10 mM); Quercetin 0.5 mM/3.0 mM; AA + Mn; Quercetin 0.5 mM/3.0 mM + Mn. Each vial represented an independent biological replicate (mixed sex); biochemical and genomic assays were performed using n = 4 vials/group. Outcomes included survival, locomotor performance (RING), redox endpoints (GSH/GSSG, H₂O₂, MDA, SOD, CAT), neuromodulators (AChE, dopamine), DNA fragmentation index (DFI), and advanced glycation end-products (AGEs). Omnibus tests (ANOVA or Kruskal-Wallis) were followed by Tukey HSD or Dunn-style pairwise comparisons; two pre-specified pooled contrasts were tested with Welch comparisons and effect sizes reported (mean differences, 95% CI, Cohen’s d); pooled p-values were adjusted (Holm, BH). Quercetin and AA produced comparable protective effects, as both preserved climbing behaviour and partially restored the redox balance versus Mn. One Tukey-adjusted pairwise result reached significance (Control vs. quercetin low + Mn for DFI, p_adj = 0.049). A pooled contrast showed reduced H₂O₂ for Quercetin (Q_all vs. AA_all: mean diff = 0.498 µmol·g⁻¹, 95% CI 0.153–0.844; Welch t(13.27) = 3.11, p = 0.0082; d ≈ 1.38), but this and other pooled signals did not survive conservative family-wise correction across all pooled tests. In conclusion, although they are overall comparable, our data suggest that quercetin may have a superior, specific effect on reducing H₂O₂ and protecting DNA integrity (DFI). These endpoints are now prioritized for confirmatory studies.

Graphical Abstract